机构：[1]State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.[2]College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.[3]College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.[4]Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510275, China.中山大学附属第二医院[5]State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.[6]Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
Beige adipocytes are a distinct type of fat cells with a thermogenic activity that have gained substantial attention as an alternative cellular anti-obesity target in humans. These cells may provide an alternative strategy for the genetic selection of pigs with reduced fat deposition. Despite the presence of beige adipocytes in piglets, the molecular signatures of porcine beige adipocytes remain unclear. Here, white and beige adipocytes from Tibetan piglets were primarily cultured and differentiated. Compared to the white adipocytes, the beige adipocytes exhibited a stronger thermogenic capacity. RNA-sequencing-based genome-wide comparative analyses revealed distinct gene expression profiles for white and beige adipocytes. In addition, two genes, integrin alpha-2 (ITGA2) and calponin 1 (CNN1), which were specifically differentially expressed in porcine beige adipocytes, were further functionally characterized using a loss-of-function approach. Our data showed that both genes were involved in differentiation and thermogenesis of porcine beige adipocytes. Collectively, these data furthered our understanding of gene expression in porcine white and beige adipocytes. Elucidating the genetic basis of beige adipogenesis in pigs will pave the way for molecular design breeding in both pigs and large animal models of human diseases.
基金：
This work was supported by the National Science Fund for Distinguished Young Scholars
(32025034), the National Key R & D Program of China (2020YFA0509503), the Laboratory of Lingnan
Modern Agriculture Project (NT2021005), and the Agricultural Science and Technology Innovation
Program (ASTIP).
第一作者机构：[1]State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.[2]College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
通讯作者：
通讯机构：[1]State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.[6]Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
推荐引用方式(GB/T 7714)：
Zhang Lilan,Hu Silu,Cao Chunwei,et al.Functional and Genetic Characterization of Porcine Beige Adipocytes.[J].CELLS.2022,11(4):doi:10.3390/cells11040751.
APA：
Zhang Lilan,Hu Silu,Cao Chunwei,Chen Chuanhe,Liu Jiali...&Wang Yanfang.(2022).Functional and Genetic Characterization of Porcine Beige Adipocytes..CELLS,11,(4)
MLA：
Zhang Lilan,et al."Functional and Genetic Characterization of Porcine Beige Adipocytes.".CELLS 11..4(2022)
