机构：[1]Institute of Digestive Surgery, Sichuan University, and Department of Gastrointestinal Surgery, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, China四川大学华西医院[2]Department of Gastrointestinal Surgery and Breast and Thyroid Surgery, Minimally Invasive Surgery, West China Fourth Hospital, Sichuan University, Chengdu, China[3]Department of Oncology and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden[4]Laboratory of Stem Cell Biology, West China Hospital, Sichuan University, Chengdu, China四川大学华西医院[5]School of Medicine, Institute of Medical Sciences, Örebro University, Örebro, Sweden
MicroRNAs (miRNAs) are known to drive the pathogenesis of colorectal cancer (CRC) via the regulation of cancer stem cells (CSCs). We studied the miRNA expression profile of primary CSCs isolated from patients with CRC (pCRCSCs). Compared to pCRCSC-derived differentiated cells, 98 differentially expressed miRNAs were identified in pCRCSCs. Target genes encoding pCRCSC-related miRNAs were identified using a combination of miRNA target databases and miRNA-mRNA regulatory networks from the same patient. The pCRCSC-related miRNA target genes were associated with pathways contributing to malignant phenotypes, including I-kappa B kinase/NF-kappa B signaling, signal transduction by p53 class mediator, Ras signaling, and cGMP-PKG signaling. The pCRCSC-related miRNA expression signature was independently associated with poor overall survival in both the training and validation cohorts. We have thus identified several pCRCSC-related miRNAs with oncogenic potential that could serve as prognostic biomarkers for CRC.
基金：
This work was supported by grants from the Sichuan
Science and Technology Program (No. 2017JY0061),
Science Fund for Creative Research Groups of the
National Natural Science Foundation of China (No.
81821002), and Swedish Cancer Foundation, Swedish
Research Council, and Oncology Clinics in Linköping
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区医学
小类|3 区细胞生物学3 区老年医学
最新[2023]版：
大类|3 区医学
小类|3 区细胞生物学3 区老年医学
第一作者：
第一作者机构：[1]Institute of Digestive Surgery, Sichuan University, and Department of Gastrointestinal Surgery, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, China[2]Department of Gastrointestinal Surgery and Breast and Thyroid Surgery, Minimally Invasive Surgery, West China Fourth Hospital, Sichuan University, Chengdu, China[3]Department of Oncology and Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden[4]Laboratory of Stem Cell Biology, West China Hospital, Sichuan University, Chengdu, China
通讯作者：
推荐引用方式(GB/T 7714)：
Fan Chuan-Wen,Lu Ran,Fang Chao,et al.Expression profile, molecular functions, and prognostic significance of miRNAs in primary colorectal cancer stem cells.[J].Aging.2021,13(8):12067-12085.doi:10.18632/aging.202914.
APA：
Fan Chuan-Wen,Lu Ran,Fang Chao,Zhang Xue-Li,Lv Zhao-Ying...&Sun Xiao-Feng.(2021).Expression profile, molecular functions, and prognostic significance of miRNAs in primary colorectal cancer stem cells..Aging,13,(8)
MLA：
Fan Chuan-Wen,et al."Expression profile, molecular functions, and prognostic significance of miRNAs in primary colorectal cancer stem cells.".Aging 13..8(2021):12067-12085
