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Aidi injection induces apoptosis of hepatocellular carcinoma cells through the mitochondrial pathway.

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资源类型：
Pubmed体系：
作者：
Lan Hai-Yue[1] ; An Pei[1] ; Liu Qiu-Ping[1] ; Chen Yu-Ying[1] ; Yu Yuan-Yuan[1] ; Luan Xin[1,*1] ; Tang Jian-Yuan[2,*2] ; Zhang Hong[1,*1] ;
机构： [1]Institute of Interdisciplinary Integrative Medicine Research, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China [2]TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China
出处：
DOI： 10.1016/j.jep.2021.114073
ISSN：

关键词： Aidi injection Liver cancer Apoptosis Mitochondria PI3K/Akt MAPK

摘要：
The incidence and mortality rates of hepatocellular carcinoma are very high all over the world, which seriously threatens human life and health. Aidi injection as a Chinese medicine preparation has a positive curative effect on hepatocellular carcinoma, but its mechanism remains unclear. The purpose of this study is to evaluate the anti-hepatocellular carcinoma effects of Aidi injection and explore its mechanism of action vitro and vivo. The main components of Aidi injection were determined by LC-MS/MS. The effects of Aidi injection on the viability of HepG2 and PLC/PRF/5 cells were detected via CCK-8 analysis and Calcein AM/PI staining. DAPI staining and flow cytometry were applied to analyze the apoptosis-induced effects of Aidi injection on hepatocellular carcinoma cells (HCCs). The growth inhibition of Aidi injection on hepatocellular carcinoma was observed in nude mice bearing PLC/PRF/5 cells. The related signal transduction and apoptosis pathways were investigated through assays for JC-1 mitochondrial membrane potential (MMP), RNA-seq, KEGG, PPI and WB. There were 12 main chemical components contained in Aidi injection, viz. cantharidin, syringin, calycosin-7-o-β-Dglucoside, isozinpidine, ginsenosides Rd, Rc, Rb1, Re, and Rg1, astragalosides II and IV, and eleutheroside E. Aidi injection significantly inhibited the proliferation of HepG2 and PLC/PLF/5 cells with IC50 of 20.66 mg/ml and 27.5 mg/ml at 48h, respectively, increased the proportion of dead cells, induced cell apoptosis, suppressed the tumor growth of nude mice bearing PLC/PLF/5 cells, reduced MMP, activated PI3K/Akt and MAPK signal transduction pathways, down-regulated the expression of p-PI3K and Bcl-xL, and up-regulated the expression of p-JNK, p-p38 and Bim. Aidi injection inhibits the growth of liver cancer probably through regulating PI3K/Akt and MAPK signal transduction pathways, inducing MMP collapse to activate the mitochondrial apoptosis pathway, and then eliciting apoptosis of HCCs. Copyright © 2021 Elsevier B.V. All rights reserved.

基金：
This work was supported by funds from National Natural Science Foundation of China (No. 81773941), National Key Subject of Drug Innovation, China (2019ZX09201005-007), National Key R & D program for key research project of modernization of traditional Chinese medicine, China (2019YFC1711602), National Science and Technology Major Project of China, China (2019ZX09201004-003-010), Program for Professor of Special Appointment (Young Eastern Scholar) at Shanghai Institutions of Higher Learning, Shanghai “Chenguang Program” of Education Commission of Shanghai Municipality (No.18CG46), and “Yangfan Program” (No.19YF1449400) of Science and Technology Commission of Shanghai Municipality.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类 | 2 区 医学
小类 | 1 区 全科医学与补充医学 2 区 植物科学 2 区 药物化学 2 区 药学
最新[2023]版：
大类 | 2 区 医学
小类 | 1 区 药物化学 1 区 全科医学与补充医学 1 区 药学 1 区 植物科学
第一作者：
第一作者机构： [1]Institute of Interdisciplinary Integrative Medicine Research, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China
共同第一作者：
通讯作者：
通讯机构： [1]Institute of Interdisciplinary Integrative Medicine Research, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China [2]TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China [*1]1200, Cailun Road, Pudong New Area, Shanghai, 201203, China [*2]39, Shi-er-qiao Road, Chengdu, 610072, Sichuan, China
推荐引用方式(GB/T 7714)：
Lan Hai-Yue,An Pei,Liu Qiu-Ping,et al.Aidi injection induces apoptosis of hepatocellular carcinoma cells through the mitochondrial pathway.[J].Journal of ethnopharmacology.2021,274:114073.doi:10.1016/j.jep.2021.114073.
APA：
Lan Hai-Yue,An Pei,Liu Qiu-Ping,Chen Yu-Ying,Yu Yuan-Yuan...&Zhang Hong.(2021).Aidi injection induces apoptosis of hepatocellular carcinoma cells through the mitochondrial pathway..Journal of ethnopharmacology,274,
MLA：
Lan Hai-Yue,et al."Aidi injection induces apoptosis of hepatocellular carcinoma cells through the mitochondrial pathway.".Journal of ethnopharmacology 274.(2021):114073

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