机构：[1]Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, No. 1, Keyuan Fourth Road, Chengdu, Sichuan 610041, P. R. China.四川大学华西医院[2]West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, No. 17, Third Section, People's South Road, Chengdu, Sichuan 610041, P. R. China.[3]Institute of Genetic Medicine, School of Life Science, Sichuan University, No. 17, Third Section, People's South Road, Chengdu, Sichuan 610041, P. R. China.
Interferon alpha 2b (IFNA2b) has been used in immunotherapy for cancers with certain success. To reduce fast diffusion of IFNA2b and consequent dose-dependent side effects, we constructed a collagen hydrogel loaded with IFNA2b fused to collagen-binding domain by using methods of tissue engineering. The fusion protein showed apoptotic activity similar to that of native IFNA2b against MCF-7 cells in vitro, but with relatively higher affinity for collagen type I. Accordingly, the former diffused out of the collagen matrix slower than the latter. Importantly, collagen hydrogels loaded with the fusion protein possessed apoptotic activity in vitro and released the engineered cytokine in a controlled manner. In addition, such hydrogels reduced tumor size and extended the survival of the mouse model with xenografted tumors, which suggested a moderate antitumor activity in vivo.
基金：
This work was supported by National Key
R&D Program of China (Grant No. 2017YFC1104702) and
National Natural Science Foundation of China (Grant No.
81473446 and 31771065).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区工程技术
小类|3 区材料科学：生物材料
最新[2023]版：
大类|2 区医学
小类|3 区材料科学：生物材料
第一作者：
第一作者机构：[1]Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, No. 1, Keyuan Fourth Road, Chengdu, Sichuan 610041, P. R. China.
通讯作者：
推荐引用方式(GB/T 7714)：
Hu Jun-Gen,Pi Jin-Kui,Jiang Yan-Lin,et al.Collagen Hydrogel Functionalized with Collagen-Targeting IFNA2b Shows Apoptotic Activity in Nude Mice with Xenografted Tumors.[J].ACS biomaterials science & engineering.2019,5(1):272-282.doi:10.1021/acsbiomaterials.8b00490.
APA：
Hu Jun-Gen,Pi Jin-Kui,Jiang Yan-Lin,Liu Xiao-Fan,Li-Ling Jesse&Xie Hui-Qi.(2019).Collagen Hydrogel Functionalized with Collagen-Targeting IFNA2b Shows Apoptotic Activity in Nude Mice with Xenografted Tumors..ACS biomaterials science & engineering,5,(1)
MLA：
Hu Jun-Gen,et al."Collagen Hydrogel Functionalized with Collagen-Targeting IFNA2b Shows Apoptotic Activity in Nude Mice with Xenografted Tumors.".ACS biomaterials science & engineering 5..1(2019):272-282
