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Proteomic Characterization of Proliferation Inhibition of Well-Differentiated Laryngeal Squamous Cell Carcinoma Cells Under Below-Background Radiation in a Deep Underground Environment.

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机构： [1]Department of Otolaryngology Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu, China, [2]Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu, China, [3]College of Water Resources and Hydropower, Sichuan University, Chengdu, China, [4]Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China, [5]Institute of Deep Earth Science and Green Energy, Shenzhen University, Shenzhen, China

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关键词： deep-underground below background radiation proteomics cell proliferation stress ribosome FD-LSC-1 cell

摘要：

Background: There has been a considerable concern about cancer induction in response to radiation exposure. However, only a limited number of studies have focused on the biological effects of below-background radiation (BBR) in deep underground environments. To improve our understanding of the effects of BBR on cancer, we studied its biological impact on well-differentiated laryngeal squamous cell carcinoma cells (FD-LSC-1) in a deep underground laboratory (DUGL). Methods: The growth curve, morphological, and quantitative proteomic experiments were performed on FD-LSC-1 cells cultured in the DUGL and above-ground laboratory (AGL). Results: The proliferation of FD-LSC-1 cells from the DUGL group was delayed compared to that of cells from the AGL group. Transmission electron microscopy scans of the cells from the DUGL group indicated the presence of hypertrophic endoplasmic reticulum (ER) and a higher number of ER. At a cutoff of absolute fold change ≥ 1.2 and p < 0.05, 807 differentially abundant proteins (DAPs; 536 upregulated proteins and 271 downregulated proteins in the cells cultured in the DUGL) were detected. KEGG pathway analysis of these DAPs revealed that seven pathways were enriched. These included ribosome (p < 0.0001), spliceosome (p = 0.0001), oxidative phosphorylation (p = 0.0001), protein export (p = 0.0001), thermogenesis (p = 0.0003), protein processing in the endoplasmic reticulum (p = 0.0108), and non-alcoholic fatty liver disease (p = 0.0421). Conclusion: The BBR environment inhibited the proliferation of FD-LSC-1 cells. Additionally, it induced changes in protein expression associated with the ribosome, gene spliceosome, RNA transport, and energy metabolism among others. The changes in protein expression might form the molecular basis for proliferation inhibition and enhanced survivability of cells adapting to BBR exposure in a deep underground environment. RPL26, RPS27, ZMAT2, PRPF40A, SNRPD2, SLU7, SRSF5, SRSF3, SNRPF, WFS1, STT3B, CANX, ERP29, HSPA5, COX6B1, UQCRH, and ATP6V1G1 were the core proteins associated with the BBR stress response in cells. Copyright © 2020 Liu, Ma, Gao, Liu, Liu, Wang, Xie, Wen, Wang, Cheng, Liu, Zou, Wu, Li and Xie.

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出版当年[2020]版：

大类 | 4 区医学

小类 | 3 区公共卫生、环境卫生与职业卫生

最新[2023]版：

大类 | 3 区医学

小类 | 3 区公共卫生、环境卫生与职业卫生

第一作者：

第一作者机构： [1]Department of Otolaryngology Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu, China, [2]Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu, China,

通讯作者：

通讯机构： [1]Department of Otolaryngology Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu, China, [2]Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu, China,

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Proteomic Characterization of Proliferation Inhibition of Well-Differentiated Laryngeal Squamous Cell Carcinoma Cells Under Below-Background Radiation in a Deep Underground Environment.

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