机构：[1]Department of Anatomy, Basic Medical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China.[2]Department of Neurosurgery, The Third People's Hospital of Chengdu, Chengdu, Sichuan 610041, P.R. China.
Glioblastoma multiforme (GBM) was one of the first cancer types systematically studied at a genomic and transcriptomic level due to its high incidence and aggressivity; however, the detailed mechanism remains unclear, even though it is known that numerous cytokines are involved in the occurrence and development of GBM. The present study aimed to determine whether the SET gene has a role in human glioblastoma carcinogenesis. A total of 32 samples, including 18 cases of glioma, 2 cases of meningioma and 12 normal brain tissue samples, were detected using the streptavidin-peroxidase method through immunohistochemistry. To reduce SET gene expression in U251 and U87MG cell lines, the RNA interference technique was used and transfection with small interfering (si)RNA of the SET gene was performed. Cell apoptosis was detected by flow cytometry, cell migration was examined by Transwell migration assay and cell proliferation was determined by Cell Counting Kit-8. SET, Bcl-2, Bax and caspase-3 mRNA and protein expression levels were detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. Positive protein expression of SET was observed in the cell nucleus, with the expression level of SET significantly higher in glioma tissues compared with normal brain tissue (P=0.001). Elevated expression of SET was significantly associated with gender (P=0.002), tumors classified as World Health Organization grade II (P=0.031), III (P=0.003) or IV (P=0.001), and moderately (P=0.031) or poorly differentiated (P=0.001) tumors. Compared with the negative and non-treatment (blank) control cells, SET gene expression was significantly inhibited (P=0.006 and P<0.001), cell apoptosis was significantly increased (P=0.001 and P<0.001), cell proliferation was significantly inhibited (P=0.002 and P=0.015), and cell migration was significantly decreased (P=0.001 and P=0.001) in siRNA-transfected U87MG-SET and U251-SET cells, respectively. In addition, mRNA and protein expression levels of Bcl-2 were significantly inhibited in U87MG-SET and U251-SET cells, while mRNA and protein expression levels of Bax and caspase-3 were significantly increased, compared with the two control groups. Thus, the current data suggests that SET may regulate the proliferation and apoptosis of glioblastoma cells by upregulating Bcl-2, and downregulating Bax and caspase-3.
基金：
the Chengdu Science and Technology Board (for research into the expression and mechanism of the SET gene in glioma).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Anatomy, Basic Medical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
通讯作者：
通讯机构：[1]Department of Anatomy, Basic Medical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, P.R. China.[2]Department of Neurosurgery, The Third People's Hospital of Chengdu, Chengdu, Sichuan 610041, P.R. China.[*1]Department of Neurosurgery, The Third People's Hospital of Chengdu, 82 Qinglong Road, Chengdu, Sichuan 610041, P.R. China[*2]Department of Anatomy, Basic Medical and Forensic Medical Institute, Sichuan University, 17 People's South Road, Chengdu, Sichuan 610041, P.R. China
推荐引用方式(GB/T 7714)：
He Kunyan,Shi Lihong,Jiang Tingting,et al.Association between SET expression and glioblastoma cell apoptosis and proliferation.[J].Oncology letters.2016,12(4):2435-2444.doi:10.3892/ol.2016.4951.
APA：
He Kunyan,Shi Lihong,Jiang Tingting,Li Qiang,Chen Yao&Meng Chuan.(2016).Association between SET expression and glioblastoma cell apoptosis and proliferation..Oncology letters,12,(4)
MLA：
He Kunyan,et al."Association between SET expression and glioblastoma cell apoptosis and proliferation.".Oncology letters 12..4(2016):2435-2444
