机构：[a]Nanjing Medical University, Nanjing, 211166, Jiangsu, PR China[b]Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China[c]Department of Neurosurgery, The Afliated Hospital of Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China[d]Department of Neurosurgery, Zigong First People’s Hospital, Zigong, 643000, Sichuan, PR China
GOLPH3, an oncoprotein, plays crucial roles in tumor etiology. Compelling evidences have demonstrated that GOLPH3 contributes to regulate tumor cell growth, migration and invasion under normal nutrient condition. However, the oncogenic activity of GOLPH3 under serum starvation remains largely unknown. In this study, we reported that GOLPH3 depletion led to marked reduction in adhesion of glioma U251 cells, particularly under serum deprivation. We found that silencing of GOLPH3 expression reduced the protein amount of ITGB1 only in serum-free medium. Further insights into the mechanism between GOLPH3 and ITGB1, we applied proteasome or lysosome inhibitor to block the degradation of ITGB1, and identified GOLPH3 silencing can prompt ITGB1 lysosomal degradation under serum starvation. Finally, we found the reductions in glioma cell adhesion and ITGB1 protein amount could be rescued by ITGB1 overexpression. Taken together, these results show that GOLPH3 contributes to the adhesion of glioma cells by regulating the lysosomal degradation of ITGB1 under serum starvation.
基金：
This study was supported by National Natural Science Foundation of China (No. 81272777; No. 81772665).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区生物学
小类|3 区生物物理4 区生化与分子生物学
最新[2023]版：
大类|3 区生物学
小类|3 区生物物理4 区生化与分子生物学
第一作者：
第一作者机构：[a]Nanjing Medical University, Nanjing, 211166, Jiangsu, PR China[b]Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China[c]Department of Neurosurgery, The Afliated Hospital of Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China
通讯作者：
通讯机构：[a]Nanjing Medical University, Nanjing, 211166, Jiangsu, PR China[b]Institute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China[c]Department of Neurosurgery, The Afliated Hospital of Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China[*1]Nanjing Medical University, Nanjing, 211166, Jiangsu, PR China.
推荐引用方式(GB/T 7714)：
Wenjian zhan,Jilun zhang,Yongchuan Luo,et al.GOLPH3 silencing inhibits adhesion of glioma U251 cells by regulating ITGB1 degradation under serum starvation.[J].Biochemical and biophysical research communications.2020,532(2):195-199.doi:10.1016/j.bbrc.2020.06.044.
APA：
Wenjian zhan,Jilun zhang,Yongchuan Luo&Rutong Yu.(2020).GOLPH3 silencing inhibits adhesion of glioma U251 cells by regulating ITGB1 degradation under serum starvation..Biochemical and biophysical research communications,532,(2)
MLA：
Wenjian zhan,et al."GOLPH3 silencing inhibits adhesion of glioma U251 cells by regulating ITGB1 degradation under serum starvation.".Biochemical and biophysical research communications 532..2(2020):195-199
