Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells.
机构:[1]Department of Medical Biology, Wannan Medical College, Wuhu, Anhui 241002[2]Tianjin Key Laboratoryof Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Center and Institute,Tianjin Medical University General Hospital, Tianjin 300052[3]Laboratory of Cancer Cell Biology,Tianjin Medical University Cancer Institute and Hospital, Hexi, Tianjin 300060[4]Department of Central Laboratory,Wannan Medical College, Wuhu, Anhui 241002[5]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center,West China Hospital, Sichuan University, Chengdu, Sichuan 610000, P.R. China四川大学华西医院
Constitutive activation of signal transducer and activator of transcription 3 (STAT3) in numerous cancers, including lung cancer, is one of the major mechanisms of tumor progression and metastasis. The authors previously reported that the metastasis suppressor non-metastasis protein 23-H1 (Nm23-H1) negatively regulates STAT3 activity by inhibiting its phosphorylation on Tyr705. Nm23-H1 is a multifunction protein that has three different kinase activities. By transfecting the five mutants that inactivated three different kinase activities respectively into Nm23-H1 deficient lung cancer cell lines, it was identified that Nm23-H1S44A (Ser44 to Ala) and Nm23-H1S120G (Ser120 to Gly) mutant forms were unable to suppress STAT3 phosphorylation on Tyr705, resulting in increased expression of fibronectin and matrix metalloproteinase-9. Notably, protein inhibitor of activated STAT3 was also involved in Nm23-H1S44A- and Nm23-H1S120G-mediated suppression of STAT3 phosphorylation. The present results indicated that Ser44 and Ser120 sites of Nm23-H1 may be responsible for its biological suppressive effects of STAT3 and tumor metastasis, which may contribute to illuminate the metastasis suppression function of Nm23-H1 in lung cancer.
基金:
The present study was supported by the National Natural Science Foundation of China (grant no. 30973364), the National Natural Science Foundation of China (grant no. 81272359) and the Key Project of Sichuan Natural Science Foundation (grant no. 06SG005-002-2).
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外文
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出版当年[2017]版:
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版:
大类|4 区医学
小类|4 区肿瘤学
第一作者:
第一作者机构:[1]Department of Medical Biology, Wannan Medical College, Wuhu, Anhui 241002[*1]Department of Medical Biology, Wannan Medical College, 22 Wenchang West Road, Wuhu, Anhui 241002, P.R. China
共同第一作者:
通讯作者:
通讯机构:[1]Department of Medical Biology, Wannan Medical College, Wuhu, Anhui 241002[2]Tianjin Key Laboratoryof Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Center and Institute,Tianjin Medical University General Hospital, Tianjin 300052[5]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center,West China Hospital, Sichuan University, Chengdu, Sichuan 610000, P.R. China[*1]Department of Medical Biology, Wannan Medical College, 22 Wenchang West Road, Wuhu, Anhui 241002, P.R. China[*2]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Center and Institute, Tianjin Medical University General Hospital, 154 Anshan Road, Heping, Tianjin 300052, P.R. China
推荐引用方式(GB/T 7714):
ZHIHAO WU,LILI GUO,JIANGNAN GE,et al.Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells.[J].Oncology letters.2017,14(2):2475-2482.doi:10.3892/ol.2017.6363.
APA:
ZHIHAO WU,LILI GUO,JIANGNAN GE,ZHIJIAN ZHANG,HUIJUN WEI&QINGHUA ZHOU.(2017).Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells..Oncology letters,14,(2)
MLA:
ZHIHAO WU,et al."Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells.".Oncology letters 14..2(2017):2475-2482