Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells.
机构：[1]Department of Medical Biology, Wannan Medical College, Wuhu, Anhui 241002[2]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Center and Institute, Tianjin Medical University General Hospital, Tianjin 300052[3]Laboratory of Cancer Cell Biology, Tianjin Medical University Cancer Institute and Hospital, Hexi, Tianjin 300060[4]Department of Central Laboratory, Wannan Medical College, Wuhu, Anhui 241002[5]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610000, P.R. China
Constitutive activation of signal transducer and activator of transcription 3 (STAT3) in numerous cancers, including lung cancer, is one of the major mechanisms of tumor progression and metastasis. The authors previously reported that the metastasis suppressor non-metastasis protein 23-H1 (Nm23-H1) negatively regulates STAT3 activity by inhibiting its phosphorylation on Tyr705. Nm23-H1 is a multifunction protein that has three different kinase activities. By transfecting the five mutants that inactivated three different kinase activities respectively into Nm23-H1 deficient lung cancer cell lines, it was identified that Nm23-H1S44A (Ser44 to Ala) and Nm23-H1S120G (Ser120 to Gly) mutant forms were unable to suppress STAT3 phosphorylation on Tyr705, resulting in increased expression of fibronectin and matrix metalloproteinase-9. Notably, protein inhibitor of activated STAT3 was also involved in Nm23-H1S44A- and Nm23-H1S120G-mediated suppression of STAT3 phosphorylation. The present results indicated that Ser44 and Ser120 sites of Nm23-H1 may be responsible for its biological suppressive effects of STAT3 and tumor metastasis, which may contribute to illuminate the metastasis suppression function of Nm23-H1 in lung cancer.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [30973364, 81272359]; Key Project of Sichuan Natural Science Foundation [06SG005-002-2]
语种：
外文
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PubmedID：
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出版当年[2017]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Medical Biology, Wannan Medical College, Wuhu, Anhui 241002[*1]Department of Medical Biology, Wannan Medical College, 22 Wenchang West Road, Wuhu, Anhui 241002, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Medical Biology, Wannan Medical College, Wuhu, Anhui 241002[2]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Center and Institute, Tianjin Medical University General Hospital, Tianjin 300052[5]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610000, P.R. China[*1]Department of Medical Biology, Wannan Medical College, 22 Wenchang West Road, Wuhu, Anhui 241002, P.R. China[*2]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Center and Institute, Tianjin Medical University General Hospital, 154 Anshan Road, Heping, Tianjin 300052, P.R. China
推荐引用方式(GB/T 7714)：
Wu Zhihao,Guo Lili,Ge Jiangnan,et al.Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells.[J].Oncology letters.2017,14(2):2475-2482.doi:10.3892/ol.2017.6363.
APA：
Wu Zhihao,Guo Lili,Ge Jiangnan,Zhang Zhijian,Wei Huijun&Zhou Qinghua.(2017).Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells..Oncology letters,14,(2)
MLA：
Wu Zhihao,et al."Two serine residues of non-metastasis protein 23-H1 are critical in inhibiting signal transducer and activator of transcription 3 activity in human lung cancer cells.".Oncology letters 14..2(2017):2475-2482
