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Autoinducer-2 of Fusobacterium nucleatum promotes macrophage M1 polarization via TNFSF9/IL-1β signaling.

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机构: [1]Department of Gastroenterology, the Affiliated Hospital of Southwest Medical University, Sichuan 646000, China [2]High Altitude Medical Research Institute, People's Hospital of Tibet Autonomous Region, Lhasa 850000, China [3]Department of Gastroenterology, The Affiliated Hospital of North Sichuan Medical College, Sichuan 637000, China
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关键词: Autoinducer-2 Colorectal cancer Fusobacterium nucleatum Gut microbiota Macrophages

摘要:
The effect of Fusobacterium nucleatum (F. nucleatum) autoinducer-2 (AI-2) on the polarization of macrophages and the underlying mechanism is not known. We investigated the effect of F. nucleatum AI-2 on the migration and polarization of cultured macrophages. We further screened AI-2-interacting proteins in macrophages using a quantitative proteomics strategy, and evaluated the expression of TNFSF9/TRAF1/p-AKT/IL-1β signaling in cultured macrophages and human colorectal cancer (CRC). The data showed that F. nucleatum AI-2 enhanced the mobility and M1 polarization of macrophages, possibly through TNFSF9/TRAF1/p-AKT/IL-1β signaling. Moreover, TNFSF9 and IL-1β expression was significantly increased in human CRCs when compared to normal colon (P < 0.05), and was associated with AI-2 concentration and increased survival. Together, our data suggested that AI-2 induced macrophage M1 polarization by activating the TNFSF9/IL-1β pathway. Thus, AI-2 may serve as a promising novel target for immunotherapy of gut microbiota-related diseases. Copyright © 2019 Elsevier B.V. All rights reserved.

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出版当年[2019]版:
大类 | 2 区 医学
小类 | 2 区 药学 3 区 免疫学
最新[2023]版:
大类 | 2 区 医学
小类 | 2 区 免疫学 2 区 药学
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第一作者机构: [1]Department of Gastroenterology, the Affiliated Hospital of Southwest Medical University, Sichuan 646000, China
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通讯机构: [3]Department of Gastroenterology, The Affiliated Hospital of North Sichuan Medical College, Sichuan 637000, China [*1]Department of Gastroenterology, The Affiliated Hospital of North Sichuan Medical College, Road Wenhua 63#, Region Shunqing, Sichuan 637000, China
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