机构:[1]Institute of Cardiovascular Disease, The 960th Hospital of Chinese PLA, Jinan, Shandong 250022中国人民解放军联勤保障部队第九六〇医院[2]Department of Clinical Microbiology and Immunology, Southwest Hospital and College of Medical Laboratory Science, The Third Military Medical University, Chongqing, Sichuan 400038[3]Department of Pediatrics, The First People's Hospital of Jining, Jining, Shandong 272000, P.R. China
Helicobacter pylori (H. pylori) infection is the major cause of chronic active gastritis and peptic ulcer disease. Upregulation of IL‑17A is associated with H. pylori infection in the gastric mucosa; however, the factors involved in the regulation of interleukin (IL)‑17A‑induced inflammatory responses in H. pylori‑associated gastritis remain unknown. MicroRNAs (miRNAs) serve as key post‑transcriptional regulators of gene expression and are associated with the H. pylori infection. The present study aimed to analyze the effects of IL‑17A on the expression of miR‑146a upon infection with H. pylori, as well as to identify the possible impact of miR‑146a dysregulation on the inflammatory response in vivo and in vitro. Reverse transcription‑quantitative polymerase chain reaction analysis was used to determine the expression levels of miR‑146a in gastric epithelial cells upon IL‑17A stimulation. The effects of miR‑146a mimics on IL‑17A‑induced inflammatory responses in SGC‑7901 cells were evaluated. The effects of miR‑146a mimics on the expression levels of IL‑1 receptor‑associated kinase 1 (IRAK1) and tumor necrosis factor receptor‑associated factor 6 (TRAF6) upon IL‑17A treatment were analyzed, and the IL‑17A‑stimulated inflammation following the silencing of IRAK1 and TRAF6 was observed. In addition, the correlation between miR‑146a and IL‑17A in human gastric mucosa with H. pylori was examined. The results indicated that IL‑17A‑induced miR‑146a may regulate the inflammatory response during the infection of H. pylori in a nuclear factor‑κB‑dependent manner. Furthermore, the expression of miR‑146a and IL‑17A are positively correlated in human gastric mucosa infected with H. pylori. These data suggested that miR‑146a may serve as a biomarker or therapeutic target in gastritis therapy.
基金:
National Natural Science Foundation of China (grant no. NSFC, 81501721) and Presidential Foundation of General Hospital of Ji'nan Military Region (grant no. 2017MS06).
第一作者机构:[1]Institute of Cardiovascular Disease, The 960th Hospital of Chinese PLA, Jinan, Shandong 250022[2]Department of Clinical Microbiology and Immunology, Southwest Hospital and College of Medical Laboratory Science, The Third Military Medical University, Chongqing, Sichuan 400038
通讯作者:
通讯机构:[1]Institute of Cardiovascular Disease, The 960th Hospital of Chinese PLA, Jinan, Shandong 250022[*1]Institute of Cardiovascular Disease, The 960th Hospital of Chinese PLA, 8 Lashan Road, Jinan, Shandong 250022, P.R. China
推荐引用方式(GB/T 7714):
NA LI,JIANLONG WANG,WENQIAN YU,et al.MicroRNA‑146a inhibits the inflammatory responses induced by interleukin‑17A during the infection of Helicobacter pylori.[J].Molecular Medicine Reports.2019,19(2):1388-1395.doi:10.3892/mmr.2018.9725.
APA:
NA LI,JIANLONG WANG,WENQIAN YU,KAI DONG,FENG YOU...&BIN QIAO.(2019).MicroRNA‑146a inhibits the inflammatory responses induced by interleukin‑17A during the infection of Helicobacter pylori..Molecular Medicine Reports,19,(2)
MLA:
NA LI,et al."MicroRNA‑146a inhibits the inflammatory responses induced by interleukin‑17A during the infection of Helicobacter pylori.".Molecular Medicine Reports 19..2(2019):1388-1395