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Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis.

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机构： [1]Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China. [2]Animal Zoology Department, Institute of Neuroscience, Kunming Medical University, Kunming 650000, Yunnan, China. [3]Department of Anesthesiology, The Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China. [4]School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, SA, Australia.

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关键词： Colorectal cancer Scutellarin Antitumor effect Genomics proteomics

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Colorectal cancer, one of the most common digestive tumors with high mortality and morbidity worldwide, currently lacks effective therapies available to improve the prognosis. This study was aimed to investigate the potency of Scutellarin against colorectal cancers, and explore the related mechanism via genomic and proteomic analysis. Cell counting kit-8 assay was employed to detect the viability of HCT-116 and RKO cell lines treated with Scutellarin. The apoptosis of HCT-116 and RKO cells after Scutellarin administration was determined by TUNEL staining and Caspase 3/7 activity. Cell cycle was detected by flow cytometry analysis. The wound healing and transwell invasion test detected the role of Scutellarin in migration and invasion of HCT-116 and RKO cells. Meanwhile, the energy metabolism and growth of tumor tissues in vivo at day 28 were observed by PET-CT after Scutellarin administration with 50 mg/kg, 100 mg/kg and 300 mg/kg into 4-week-old nude mice. Blood routine and liver functions were also detected to evaluate the side effect of Scutellarin. Furthermore, the disease and function classifications which the differentially expressed genes and proteins involved after Scutellarin treatment were determined by genomic and proteomic analysis respectively. The Scutellarin inhibited the migration and increased apoptosis of HCT-116 and RKO cell lines. Besides, Scutellarin treatment substantially decreased the growth and volume of colorectal tumors in nude mice without side effects on the blood routine and liver function. The differentially expressed genes in RKO cells after Scutellarin administration were mainly enriched in cell death and survival, organismal injury and abnormalities, and cancer. In addition, forty-seven upregulated and twenty-nine downregulated proteins were identified. Functional clustering analysis exhibited enriched biological processes, cellular components, molecular functions and related pathways of these proteins in cellular metabolic. Then protein-protein interactions analysis showed the regulatory relationship among these differentially expressed proteins. Taken together, the present findings revealed that Scutellarin exerted significant antitumor effect with no side effects in the blood and liver by regulating various important molecules in tumor proliferation, apoptosis and metastasis. © The Author(s) 2020.

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出版当年[2020]版：

大类 | 3 区医学

小类 | 3 区全科医学与补充医学 3 区药学

最新[2023]版：

大类 | 3 区医学

小类 | 2 区全科医学与补充医学 2 区药学

第一作者：

第一作者机构： [1]Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China. [3]Department of Anesthesiology, The Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, People’s Republic of China. [4]School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, SA, Australia.

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通讯作者：

通讯机构： [1]Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China. [2]Animal Zoology Department, Institute of Neuroscience, Kunming Medical University, Kunming 650000, Yunnan, China.

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Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis.

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