Human telomerase reverse transcriptase(hTERT) activity was detected in human nasopharyngeal carcinoma cell(CNE) but not in human normal lung fibroblast(CCD-11Lu). Recombinant adenoviruses Ad-CMV-TK-enh and Ad-hTERT-TK-enh were constructed and infected into normal fibroblasts and nasopharyngeal carcinoma cells. Ad-CMv-TK-enh with 100 [mu mol/L of ganciclovir(GCV) caused 87% of CCD-11Lu cells death and 91% of CNE cells death, Ad-hTERT-TK-enh with 100 mu mol/L of GCV caused 24% of CCD-11Lu cells death and 79% of CNE cells death. These results indicate that the Ad-hTERT-TK-enh with GCV may be a useful method in suppressing tumor growth in targeted nasopharyngeal carcinoma gene therapy.
基金：
Agency of Science and Technology of Sichuan Province, China（No.2008JY0020）； the National Natural Science Foundation of China（No.30872396
第一作者机构：[1]Sichuan Tumor Hosp, Dept Radiotherapy, Chengdu 610041, Peoples R China[3]Jilin Univ, Coll Life Sci, Vaccine Res Ctr, Changchun 130012, Peoples R China
通讯作者：
通讯机构：[1]Sichuan Tumor Hosp, Dept Radiotherapy, Chengdu 610041, Peoples R China[2]Sichuan Tumor Hosp, Dept Mol Pharmacol, Chengdu 610041, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Yu,Yu Xiang-hui,Zha Xiao,et al.Adenovirus-mediated HSV-TK Gene Therapy Using hTERT Promoter in CNE Cells in vitro[J].CHEMICAL RESEARCH IN CHINESE UNIVERSITIES.2009,25(1):60-63.
APA：
Zhang Yu,Yu Xiang-hui,Zha Xiao&Kong Wei.(2009).Adenovirus-mediated HSV-TK Gene Therapy Using hTERT Promoter in CNE Cells in vitro.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,25,(1)
MLA：
Zhang Yu,et al."Adenovirus-mediated HSV-TK Gene Therapy Using hTERT Promoter in CNE Cells in vitro".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 25..1(2009):60-63
