To investigate whether telomerase activity, human telomerase RNA (HTR) and human telomerase reverse transcriptase (HTERT) expression were associated with tumor development in lung cancer and whether telomerase is regulated at gene level or transcriptional level.
Expression of HTR and HTERT was detected by reverse transcription-polymerase chain reaction (RT-PCR) in 68 human lung cancer and in 68 adjacent-neoplatic lung tissues. And telomerase activity was examined by a quantitative telomeric repeat amplification protocol (TRAP).
In 68 lung cancer tissues, telomerase activity, HTR and HTERT were expressed in 79%, 98.5% and 91.2% respectively, whereas all adjacent non-neoplastic lung tissues were telomerase negative. Most normal lung tissues expressed HTR (91.2%) and HTERT was detected in only 7 of 68 non-neoplastic tissues.
The relatively high frequency of telomerase activity in lung cancer whereas the detection of no telomerase activity in normal lung tissues suggested that telomerase may play an important role in tumorigenesis of lung cancer. Compared to HTR, HTERT expression was better associated with telomerase activity with a concordance of 88.9%. Telomerase activity may be regulated at transcription level or translation level.
基金：
National Natural Sciences Foundation of China （No 395 70 392 ）
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2002]版：
无
最新[2023]版：
大类|3 区医学
小类|3 区医学：内科
第一作者：
第一作者机构：[1]Cancer Institute, First Hospital of Huaxi Medical Center, Sichuan University, Sichuan 610041, China.
通讯作者：
通讯机构：[1]Cancer Institute, First Hospital of Huaxi Medical Center, Sichuan University, Sichuan 610041, China.[*1]Cancer InstituteWest China University of Medical SciencesChengdu 610041China
推荐引用方式(GB/T 7714)：
Chen Weiwei,Xiong Xiaoxiong,Zhou Hongyuan,et al.端粒酶、端粒酶RNA组分和端粒酶催化亚单位在肺癌中的表达（英文）[J].Chinese Medical Journal.2002,115(2):290-2.
APA：
Chen Weiwei,Xiong Xiaoxiong,Zhou Hongyuan&Zhou Qinghua.(2002).端粒酶、端粒酶RNA组分和端粒酶催化亚单位在肺癌中的表达（英文）.Chinese Medical Journal,115,(2)
MLA：
Chen Weiwei,et al."端粒酶、端粒酶RNA组分和端粒酶催化亚单位在肺癌中的表达（英文）".Chinese Medical Journal 115..2(2002):290-2
