机构：[1]State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, People’s Republic of China四川大学华西医院[2]Department of Chemotherapy, Sichuan Cancer Hospital, Chengdu, Sichuan, People’s Republic of China四川省肿瘤医院
Vesicular stomatitis virus (VSV) matrix protein (MP) can induce in vitro apoptosis of tumor cells in the absence of other viral components. Here, the antitumor activity of VSV-MP against lung adenocarcinoma was investigated in vivo. A pVAX-plasmid DNA encoding VSV-MP and control empty vectors (pVAX) were constructed and wrapped-up with liposome. A549 and Spc-A1 human lung adenocarcinoma cells were transfected with liposomal-VSV-MP (Lip-MP) or Lip-pVAX and then examined for cell viability or apoptosis using Hoechst/propidium iodide staining by flow cytometry, and further demonstrated by caspase/poly ADP-ribose polymerase (PARP) cleavage analysis. For the in vivo study, A549 and Spc-A1 lung carcinoma models in nude mice were established and randomly assigned into three groups to receive eight 2-weekly intravenous administrations of medium alone as control, Lip-pVAX or Lip-MP, respectively. Subsequently, Lip-MP significantly reduced tumor growth and prolonged the survival of tumor-bearing mice compared with Lip-pVAX and control agents (P<0.05), with much higher apoptosis index of both in vivo and in vitro tumor cells, respectively (P<0.05). In addition, in vivo antitumoral effect was associated with natural killer-(NK) cell congregation without evidence of toxicity. These observations suggest that systemically delivering Lip-MP has a specific dual antitumor activity in human lung adenocarcinoma by inducing apoptosis and possibly stimulating NK-cell responses, it may provide a clue for developing new therapeutic approaches against human lung adenocarcinoma. Cancer Gene Therapy (2012) 19, 101-109; doi: 10.1038/cgt.2011.71; published online 4 November 2011
基金：
National 973 ProjectNational Basic Research Program of China [2010CB529900]; National Natural Science FoundationNational Natural Science Foundation of China [30973452]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2012]版：
大类|3 区医学
小类|3 区生物工程与应用微生物3 区遗传学3 区医学：研究与实验3 区肿瘤学
最新[2023]版：
大类|3 区医学
小类|2 区生物工程与应用微生物3 区遗传学3 区医学：研究与实验4 区肿瘤学
JCR分区：
出版当年[2012]版：
Q2ONCOLOGYQ2GENETICS & HEREDITYQ2MEDICINE, RESEARCH & EXPERIMENTALQ2BIOTECHNOLOGY & APPLIED MICROBIOLOGY
最新[2023]版：
Q1BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ1GENETICS & HEREDITYQ1MEDICINE, RESEARCH & EXPERIMENTALQ1ONCOLOGY
第一作者机构：[1]State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, People’s Republic of China[2]Department of Chemotherapy, Sichuan Cancer Hospital, Chengdu, Sichuan, People’s Republic of China
通讯作者：
通讯机构：[1]State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan, People’s Republic of China[*1]West China Medical School, Sichuan University, Keyuan Road 4, Chengdu, Sichuan 610041, People’s Republic of China
推荐引用方式(GB/T 7714)：
Jing X-M,Wen Y-J,Shi W.,et al.VSV-MP gene therapy strategy inhibits tumor growth in nude mice model of human lung adenocarcinoma[J].CANCER GENE THERAPY.2012,19(2):101-109.doi:10.1038/cgt.2011.71.
APA：
Jing, X-M,Wen, Y-J,Shi, W.,Tang, Q-Q,Li, J.&Chen, X-C.(2012).VSV-MP gene therapy strategy inhibits tumor growth in nude mice model of human lung adenocarcinoma.CANCER GENE THERAPY,19,(2)
MLA：
Jing, X-M,et al."VSV-MP gene therapy strategy inhibits tumor growth in nude mice model of human lung adenocarcinoma".CANCER GENE THERAPY 19..2(2012):101-109
