Dual tumor-targeted poly(lactic-co-glycolic acid)-polyethylene glycol-folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery
机构：[1]Key Laboratory for Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing,[2]Institute of Laboratory Animals, Sichuan Academy of Medical Science, Sichuan Provincial People’s Hospital, Chengdu,四川省人民医院[3]Institute of Life Sciences, Wenzhou University, Wenzhou, 4School of Medicine, Wuhan University, Wuhan, China
Further specific target-ability development of biodegradable nanocarriers is extremely important to promote their security and efficiency in antitumor drug-delivery applications. In this study, a facilely prepared poly(lactic-co-glycolic acid) (PLGA)-polyethylene glycol (PEG)-folic acid (FA) copolymer was able to self-assemble into nanoparticles with favorable hydrodynamic diameters of around 100 nm and negative surface charge in aqueous solution, which was expected to enhance intracellular antitumor drug delivery by advanced dual tumor-target effects, ie, enhanced permeability and retention induced the passive target, and FA mediated the positive target. Fluorescence-activated cell-sorting and confocal laser-scanning microscopy results confirmed that doxorubicin (model drug) loaded into PLGA-PEG-FA nanoparticles was able to be delivered efficiently into tumor cells and accumulated at nuclei. In addition, all hemolysis, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, and zebrafish-development experiments demonstrated that PLGA-PEG-FA nanoparticles were biocompatible and secure for biomedical applications, even at high polymer concentration (0.1 mg/mL), both in vitro and in vivo. Therefore, PLGA-PEG-FA nanoparticles provide a feasible controlled-release platform for secure and efficient antitumor drug delivery.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [51603023]; Fundamental Research Funds for the National Key RD Project [2016YFC1102305]; Fundamental Research Funds for Central UniversitiesFundamental Research Funds for the Central Universities [106112016CDJXY230002]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M602656, 2017T100682]; Chongqing Research Program of Basic research and Frontier Technology [cstc2017jcyjAX0186]; Chongqing Postdoctoral Scientific Research Foundation [Xm2016011]
第一作者机构：[1]Key Laboratory for Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing,[2]Institute of Laboratory Animals, Sichuan Academy of Medical Science, Sichuan Provincial People’s Hospital, Chengdu,
共同第一作者：
通讯作者：
通讯机构：[1]Key Laboratory for Biorheological Science and Technology, Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College, Chongqing University, Chongqing,[*1]Bioengineering College, Chongqing University, 174 Shazheng Street, Shapingba Qu, Chongqing, 400030, China
推荐引用方式(GB/T 7714)：
Jia Chen,Qi Wu,Li Luo,et al.Dual tumor-targeted poly(lactic-co-glycolic acid)-polyethylene glycol-folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery[J].INTERNATIONAL JOURNAL OF NANOMEDICINE.2017,12:5745-5760.doi:10.2147/IJN.S136488.
APA：
Jia Chen,Qi Wu,Li Luo,Yi Wang,Yuan Zhong...&Gui-Xue Wang.(2017).Dual tumor-targeted poly(lactic-co-glycolic acid)-polyethylene glycol-folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery.INTERNATIONAL JOURNAL OF NANOMEDICINE,12,
MLA：
Jia Chen,et al."Dual tumor-targeted poly(lactic-co-glycolic acid)-polyethylene glycol-folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery".INTERNATIONAL JOURNAL OF NANOMEDICINE 12.(2017):5745-5760
医学