lncRNA TUG1-Mediated Mir-142-3p Downregulation Contributes to Metastasis and the Epithelial-to-Mesenchymal Transition of Hepatocellular Carcinoma by Targeting ZEB1
机构：[1]Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, Chengdu,四川大学华西医院[2]Department of Oncology, Sichuan Cancer Hospital & Institute, Chengdu,四川省肿瘤医院[3]Department of Pathology, Nanjing First Hospital, Nanjing Medical University, Nanjing,[4]Traditional Chinese Medicine Academy of Heilongjiang, Harbin, China
Background/Aims: MicroRNA-142-3p (miR-142-3p) is dysregulated in many malignancies and may function as a tumor suppressor or oncogene in tumorigenesis and tumor development. However, few studies have investigated the clinical significance and biological function of miR-142-3p in hepatocellular carcinoma (HCC). Methods: The expression levels of taurine upregulated gene 1 (TUG1), miR-142-3p, and zinc finger E-box-binding homeobox 1 (ZEB1) were evaluated in HCC tissues and cell lines by quantitative real-time PCR. MTT and colony formation assays were used to detect cell proliferation ability, transwell assays were used to assess cell migration and invasion, and luciferase reporter assays were used to examine the interaction between the long noncoding RNA TUG1 and miR-142-3p. Tumor formation was evaluated through in vivo experiments. Results: miR-142-3p was significantly downregulated in HCC tissues, but TUG1 was upregulated in HCC tissues. Knockdown of TUG1 and upregulation of miR-142-3p inhibited cell proliferation, cell migration, cell invasion, and the epithelial-mesenchymal transition (EMT). miR-142-3p was found to be a prognostic factor of HCC, and the mechanism by which TUG1 upregulated ZEB1 was via direct binding to miR-142- 3p. In vivo assays showed that TUG1 knockdown suppressed cell proliferation and the EMT in nude mice. Conclusion: The results of this study suggest that the TUG1/miR-142-3p/ZEB1 axis contributes to the formation of malignant behaviors in HCC. (C) 2018 The Author(s) Published by S. Karger AG, Basel
基金：
National Natural Science Foundation of China (Grant Nos. 81374014 and 81672430), the Zhejiang Provincial Science and Technology Projects (Grant Nos. 2015C33264, 2017C33212, and 2017C33213), and the Zhejiang Provincial Medical and Healthy Science and Technology Projects (Grant Nos. 2013KYA228 and 2016KYA180).
第一作者机构：[1]Department of Hematology, Hematology Research Laboratory, West China Hospital, Sichuan University, Chengdu,
共同第一作者：
通讯作者：
通讯机构：[4]Traditional Chinese Medicine Academy of Heilongjiang, Harbin, China[*1]Traditional Chinese Medicine Academy of Heilongjiang No.142 Xiangshun Street, Xiangfang district, Harbin, 150036, Heilongjiang (China)
推荐引用方式(GB/T 7714)：
He Chuan,Liu Zhigang,Jin Li,et al.lncRNA TUG1-Mediated Mir-142-3p Downregulation Contributes to Metastasis and the Epithelial-to-Mesenchymal Transition of Hepatocellular Carcinoma by Targeting ZEB1[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2018,48(5):1928-1941.doi:10.1159/000492517.
APA：
He, Chuan,Liu, Zhigang,Jin, Li,Zhang, Fang,Peng, Xinhao...&Cai, XiaoJun.(2018).lncRNA TUG1-Mediated Mir-142-3p Downregulation Contributes to Metastasis and the Epithelial-to-Mesenchymal Transition of Hepatocellular Carcinoma by Targeting ZEB1.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,48,(5)
MLA：
He, Chuan,et al."lncRNA TUG1-Mediated Mir-142-3p Downregulation Contributes to Metastasis and the Epithelial-to-Mesenchymal Transition of Hepatocellular Carcinoma by Targeting ZEB1".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 48..5(2018):1928-1941
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