机构：[1]Department of Urology, Nanyang Second People's Hospital of Henan Province, Nanyang, 473000, Henan, China[2]Sichuan Cancer Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, Sichuan, China四川省人民医院四川省肿瘤医院[3]Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, 201403, China[4]Department of Pharmaceutical Botany, School of Pharmacy, The Second Military Medical University, Shanghai, 200433, China
Objectives: Prostate cancer, one of the most frequently diagnosed tumors of men, leads to poor quality of life. Previous studies have shown that breviscapine (BRE) exerts therapeutic activity in malignant tumors. However, the role and mechanism of BRE exhibit an anti-tumor effect on prostate cancer are largely unknown. Methods: The mRNA and protein levels in prostate cancer tissues and cell lines were measured using RT-qPCR, western blot, and immunohistochemical staining, respectively. Cell proliferation, invasion, and migration in both PC3 and DU145 cells were evaluated using CCK-8 and Transwell assay. The effect of BRE on cell proliferation and metastasis by regulating the PAQR4-mediated PI3K/Akt pathway in vitro and in vivo was determined. Results: PAQR4 was significantly overexpressed in prostate cancer tissues and cell lines, which was positively correlated with poor prognosis. Knockdown of PAQR4 inhibited the proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT) of both PC3 and DU145 cells. Mechanistically, BRE treatment significantly suppressed the malignant biological behavior of both prostate cancer cells by downregulating PAQR4 and blocking the PI3K/Akt pathway. In vivo experiments, BRE administration remarkably inhibited tumor growth and metastasis in a xenograft model of prostate cancer. Conclusion: Our findings revealed that BRE exerts anti-tumor and anti-metastasis roles in prostate cancer by inhibiting PAQR4-mediated PI3K/Akt pathway, which provides a new therapeutic agent for prostate cancer clinical treatment.
基金：
Youth Talent Development Plan of Shanghai Municipal Agricultural System of China [20180117]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区医学
小类|2 区医学：研究与实验2 区药学
最新[2023]版：
大类|2 区医学
小类|1 区药学2 区医学：研究与实验
JCR分区：
出版当年[2020]版：
Q1PHARMACOLOGY & PHARMACYQ1MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ1PHARMACOLOGY & PHARMACY
第一作者机构：[1]Department of Urology, Nanyang Second People's Hospital of Henan Province, Nanyang, 473000, Henan, China
共同第一作者：
通讯作者：
通讯机构：[3]Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Shanghai, 201403, China[4]Department of Pharmaceutical Botany, School of Pharmacy, The Second Military Medical University, Shanghai, 200433, China[*1]No. 325, Guohe Road, Shanghai, 200433, China.[*2]No. 1000, Jinqi Road, Shanghai, 201403, China.
推荐引用方式(GB/T 7714)：
Ye Jiwei,Gao Mingquan,Guo Xinwu,et al.Breviscapine suppresses the growth and metastasis of prostate cancer through regulating PAQR4-mediated PI3K/Akt pathway[J].BIOMEDICINE & PHARMACOTHERAPY.2020,127:doi:10.1016/j.biopha.2020.110223.
APA：
Ye, Jiwei,Gao, Mingquan,Guo, Xinwu,Zhang, Henan&Jiang, Fuchun.(2020).Breviscapine suppresses the growth and metastasis of prostate cancer through regulating PAQR4-mediated PI3K/Akt pathway.BIOMEDICINE & PHARMACOTHERAPY,127,
MLA：
Ye, Jiwei,et al."Breviscapine suppresses the growth and metastasis of prostate cancer through regulating PAQR4-mediated PI3K/Akt pathway".BIOMEDICINE & PHARMACOTHERAPY 127.(2020)
医学