Simultaneous quantification of imatinib and its main metabolite N-demethyl-imatinib in human plasma by liquid chromatography-tandem mass spectrometry and its application to therapeutic drug monitoring in patients with gastrointestinal stromal tumor
The aim of this study was to improve and validate a more stable and less time-consuming method based on liquid chromatography and tandem mass spectrometry (LC- MS/MS) for the quantitative measurement of imatinib and its metabolite N-demethyl-imatinib (NDI) in human plasma. Separation of analytes was performed on a Waters XTerra RP18 column (50x2.1mm i.d., 3.5m) with a mobile phase consisting of methanol-acetonitrile-water (65:20:15, v/v/v) with 0.05% formic acid at a flow-rate of 0.2mL/min. The Quattro MicroTM triple quadruple mass spectrometer was operated in the multiple-reaction-monitoring mode via positive electrospray ionization interface using the transitions m/z 494.0394.0 for imatinib, m/z 479.6394.0 for NDI and m/z 488.2394.0 for IS. The method was linear over 0.01-10g/mL for imatinib and NDI. The intra- and inter-day precisions were all <15% in terms of relative standard deviation, and the accuracy was within +/- 15% in terms of relative error for both imatinib and NDI. The lower limit of quantification was identifiable and reproducible at 10ng/mL. The method was sensitive, specific and less time-consuming and it was successfully applied in gastrointestinal stromal tumor patients treated with imatinib.
基金：
National Science Foundation of ChinaNational Natural Science Foundation of China [81473283, 81372474, 81173131, 81573507, 81320108027]; Science and Technology Ministry of ChinaMinistry of Science and Technology, China [2012ZX09506001-004]; Major Scientific and Technological Project of Guangdong Province [2011A080300001]; Science and Technology Program of Guangzhou [2014 J4100179]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
最新[2023]版：
大类|4 区医学
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
第一作者：
第一作者机构：[1]Sun Yat Sen Univ, Inst Clin Pharmacol, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China;
通讯作者：
通讯机构：[1]Sun Yat Sen Univ, Inst Clin Pharmacol, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, State Key Lab Oncol South China, Dept Gastr & Pancreat Surg, Collaborat Innovat Ctr Canc Med,Canc Ctr, Guangzhou, Guangdong, Peoples R China;[4]Dept Gastr & Pancreat Surg, Collaborat Innovat, State Key Lab Oncol S China, Guangzhou, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Zhuang Wei,Qiu Hai-Bo,Chen Xin-Meng,et al.Simultaneous quantification of imatinib and its main metabolite N-demethyl-imatinib in human plasma by liquid chromatography-tandem mass spectrometry and its application to therapeutic drug monitoring in patients with gastrointestinal stromal tumor[J].BIOMEDICAL CHROMATOGRAPHY.2017,31(12):-.doi:10.1002/bmc.4022.
APA：
Zhuang, Wei,Qiu, Hai-Bo,Chen, Xin-Meng,Yuan, Xiu-Hong,Yang, Li-Fang...&Zhou, Zhi-Wei.(2017).Simultaneous quantification of imatinib and its main metabolite N-demethyl-imatinib in human plasma by liquid chromatography-tandem mass spectrometry and its application to therapeutic drug monitoring in patients with gastrointestinal stromal tumor.BIOMEDICAL CHROMATOGRAPHY,31,(12)
MLA：
Zhuang, Wei,et al."Simultaneous quantification of imatinib and its main metabolite N-demethyl-imatinib in human plasma by liquid chromatography-tandem mass spectrometry and its application to therapeutic drug monitoring in patients with gastrointestinal stromal tumor".BIOMEDICAL CHROMATOGRAPHY 31..12(2017):-
