机构：[1]Department of Structural & Cellular Biology, Tulane University, New Orleans, LA, USA[2]Department of Orthopaedic Surgery, Tulane University, New Orleans, LA, USA[3]Tulane Cancer Center and Louisiana Cancer Research Consortium, Tulane University, New Orleans, LA, USA[4]Tulane Center for Stem Cell Research and Regenerative Medicine, Tulane University, New Orleans, LA, USA[5]Tulane Center for Aging, Tulane University, New Orleans, LA, USA[6]Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China[7]Department of Gastroenterological Surgery, Tangshan People’s Hospital, Tangshan, China[8]Lung Cancer Research Institute and Cancer Center, Guangdong General Hospital, Guangzhou, China肿瘤治疗中心研究所肺肿瘤科广东省肺癌研究所广东省人民医院
Organoids mimic the architecture and functions of a small organ. Organoid culture technique has been rapidly accepted by all research communities during the past decade to study stem cells, organ development and function, and patient-specific diseases. A protocol for organoid culture of human and mouse prostate epithelial and cancer tissues has been reported. However, organoid culture of the commonly used human prostate cancer cell lines has yet to be established. We followed the published protocol and performed organoid culture of LNCaP and C4-2B cells in MatrigelTM and organoid culture medium for 14 days. We found that both LNCaP and C4-2B cell lines formed organoids that presented glandular structures. The cells within the organoids were androgen receptor-positive adenocarcinoma cells, but not p63-positive basal cells. The cells in the organoids responded to interleukin-17A treatment differently from the cells in the monolayer culture. The present study suggests that LNCaP and C4-2B cells are able to form organoids under the defined organoid culture conditions.
基金：
Z.Y. was supported partially by National Institutes
of Health (R01CA174714 and P20-
GM103518), Department of Defense (W81-
XWH-14-1-0050, W81XWH-14-1-0149, W81XWH-
14-1-0458 (PI: Feng Chen; Co-I: Z.Y.), and
W81XWH-15-1-0444), the Developmental Fund
of Tulane Cancer Center (TCC), Louisiana
Cancer Research Consortium (LCRC) Fund, and
Tulane’s Institute of Integrated Engineering for
Health and Medicine (TI2EHM). This work was
supported by the Carol Lavin Bernick Faculty
Grant, donated by the Carol Lavin Bernick
Family Foundation to Tulane University.
语种：
外文
被引次数：
WOS：
PubmedID：
第一作者：
第一作者机构：[1]Department of Structural & Cellular Biology, Tulane University, New Orleans, LA, USA[6]Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China
通讯作者：
通讯机构：[1]Department of Structural & Cellular Biology, Tulane University, New Orleans, LA, USA[2]Department of Orthopaedic Surgery, Tulane University, New Orleans, LA, USA[3]Tulane Cancer Center and Louisiana Cancer Research Consortium, Tulane University, New Orleans, LA, USA[4]Tulane Center for Stem Cell Research and Regenerative Medicine, Tulane University, New Orleans, LA, USA[5]Tulane Center for Aging, Tulane University, New Orleans, LA, USA[*1]Department of Structural & Cellular Biology, Tulane University School of Medicine, 1430 Tulane Ave Mailbox 8649, New Orleans, LA 70112, USA
推荐引用方式(GB/T 7714)：
Lin Ma,Jingwu Li,Qiang Nie,et al.Organoid culture of human prostate cancer cell lines LNCaP and C4-2B.[J].American journal of clinical and experimental urology.2017,5(3):25-33.
APA：
Lin Ma,Jingwu Li,Qiang Nie,Qiuyang Zhang,Sen Liu...&Zongbing You.(2017).Organoid culture of human prostate cancer cell lines LNCaP and C4-2B..American journal of clinical and experimental urology,5,(3)
MLA：
Lin Ma,et al."Organoid culture of human prostate cancer cell lines LNCaP and C4-2B.".American journal of clinical and experimental urology 5..3(2017):25-33
