Introduction: This study was designed to investigate the potential function of the activating protein 2 alpha (AP-2 alpha) gene in controlling the proliferation and apoptosis of gastric cancer. Material and methods: Gastric cancer cell line MCG-803 cells and normal cell line GES-1 cells were selected to transfect pcDNA3.1(+)-AP-2 alpha and pcDNA3.1(+) plasmids, respectively. Both mRNA and protein levels of AP-2 alpha in each group transfected with the pcDNA3.1(+)-AP-2 alpha plasmids were up-regulated after 48 h by real-time PCR and Western blotting analysis, leading to marked proliferation inhibition and significant cell cycle arrest. Results: pcDNA3.1(+)-AP-2 alpha reduced tumor tissue growth in a subcutaneous tumor gastric carcinoma nude mouse model. Protein over-expression of AP-2 alpha in the nude mouse model was accompanied by down-regulation of Blc-2 and ErbB2, resulting in the up-regulation of caspase-3, -8, and -9, ER alpha and p21(WAF1/CIP1). Conclusions: The reintroduction of the AP-2a gene by pcDNA3.1 could inhibit gastric tumor growth in vitro and in vivo, which may be an alternative future therapeutic molecular target for human gastric cancer.
基金：
National Natural Science Foundation of China [81172080, 81401771, 81201773]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20120171120114]; Science and Technology Project of Zhejiang Province [2014C33236]; China Postdoctoral Science Foundation [2014M560296]; Science and Technology Projects of Guangdong Province [2011B031800181]; Shanghai Municipal Education Commission [15ZZ006]; Natural Science Foundation of Shanghai [15ZR1405000]; science and technology project of Fujian Province [2014J01284]
语种：
外文
被引次数：
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PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|3 区医学：内科
最新[2023]版：
大类|4 区医学
小类|3 区医学：内科
第一作者：
第一作者机构：[1]Sun Yat Sen Univ, Dept Gastr Surg, Canc Ctr, State Key Lab Oncol South China,Collaborat Innova, Guangzhou, Guangdong, Peoples R China;[2]Fujian Med Univ, Prov Clin Med Coll, Fujian Prov Hosp, Dept Gastrointestinal Surg, Fuzhou, Fujian, Peoples R China;
通讯作者：
通讯机构：[6]Fudan Univ, Huashan Hosp, Dept Orthoped Sports Med, Shanghai, Peoples R China;[7]Sun Yat Sen Univ, Dept Gastr & Pancreat Surg, Canc Ctr, State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Zeng Changqing,Liu Zhimin,Zhang Jian,et al.Functions of the AP-2 alpha gene in activating apoptosis and inhibiting proliferation of gastric cancer cells both in vitro and in vivo[J].ARCHIVES OF MEDICAL SCIENCE.2017,13(6):1255-1261.doi:10.5114/aoms.2017.71064.
APA：
Zeng, Changqing,Liu, Zhimin,Zhang, Jian,Fang, Hongwei,Fang, Cheng...&Wang, Wei.(2017).Functions of the AP-2 alpha gene in activating apoptosis and inhibiting proliferation of gastric cancer cells both in vitro and in vivo.ARCHIVES OF MEDICAL SCIENCE,13,(6)
MLA：
Zeng, Changqing,et al."Functions of the AP-2 alpha gene in activating apoptosis and inhibiting proliferation of gastric cancer cells both in vitro and in vivo".ARCHIVES OF MEDICAL SCIENCE 13..6(2017):1255-1261
