机构：[1]Department of Clinical Pharmacy, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China临床药学部临床药学部四川省人民医院四川省肿瘤医院[2]Personalized Drug Therapy Key Laboratory of Sichuan Province, Chengdu, China[3]School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China
CRISPR technology has been used to revolutionize various facets of life sciences because of its potent gene editing capabilities. In particular, CRISPR technology is anticipated to be used to cure congenital disorders, and malignant cancers brought on by gene mutation. In this article, we introduce a Split-Cas9 system, in which Cas9 protein is split into two or more parts and recombined in cells to function specific induction circumstances. Split-Cas9 system can improve the therapeutic index of CRISPR technology by splitting Cas9 proteins into small fragments, thus enhancing their compatibility with virus vectors and precise temporal and spatial control. This paper examined the combination mode of Split-Cas9 system, contrasted the differences in its split sites and activity efficiency, and discussed the use and clinical transformation in vivo and in vitro.
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|3 区医学
小类|2 区生物工程与应用微生物3 区遗传学3 区医学：研究与实验
最新[2023]版：
大类|3 区医学
小类|2 区生物工程与应用微生物3 区遗传学3 区医学：研究与实验
JCR分区：
出版当年[2023]版：
Q1GENETICS & HEREDITYQ2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ2MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1GENETICS & HEREDITYQ2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ2MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of Clinical Pharmacy, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China[2]Personalized Drug Therapy Key Laboratory of Sichuan Province, Chengdu, China
通讯作者：
通讯机构：[1]Department of Clinical Pharmacy, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, China[2]Personalized Drug Therapy Key Laboratory of Sichuan Province, Chengdu, China
推荐引用方式(GB/T 7714)：
Liu Zhixi,Huang Lu,Deng Han,et al.Characteristics, Recombination methods and Applications progresses of Split-Cas9 System[J].HUMAN GENE THERAPY.2023,34(13-14):594-604.doi:10.1089/hum.2022.223.
APA：
Liu Zhixi,Huang Lu,Deng Han,Chen Yan&Xiao Hongtao.(2023).Characteristics, Recombination methods and Applications progresses of Split-Cas9 System.HUMAN GENE THERAPY,34,(13-14)
MLA：
Liu Zhixi,et al."Characteristics, Recombination methods and Applications progresses of Split-Cas9 System".HUMAN GENE THERAPY 34..13-14(2023):594-604
