机构：[1]From the *Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Sichuan, People's Republic of China四川大学华西医院[2]Department of Gastroenterological Surgery, West China Hospital, Sichuan University, Sichuan, People's Republic of China.四川大学华西医院
The aim of the study was to explore the potential role of myeloid differentiation factor 88 (MyD88), which acts as an adaptor in the TLR4 signalling pathway, in immune responses of the pancreatic duct during acute pancreatitis.Primary cultures of pancreatic duct epithelial cells from Wistar rats and cultures of the pancreatic ductal ARIP cell line were treated with lipopolysaccharide (LPS), and expression of toll-like receptor 4 mRNA was determined using real-time PCR, expression of MyD88 protein using Western blot, and levels of inflammatory cytokines using enzyme-linked immunosorbent assay. These experiments were repeated using ARIP cells in which MyD88 expression was stably knocked down.Toll-like receptor 4 and MyD88 expression were similar between pancreatic duct epithelial cells and ARIP cells after LPS stimulation. Myeloid differentiation factor 88 knockdown led to significantly lower levels of inflammatory cytokines after LPS induction in ARIP cells.Myeloid differentiation factor 88 knockdown attenuates LPS-induced inflammatory responses in pancreatic ductal cells, suggesting that the MyD88 pathway plays a critical role in their immune defense activity.
基金：
National Natural Science
Foundation of China (NSFC key project 30830100 and projects 81170439,
81470886, and 81500486).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区医学
小类|3 区胃肠肝病学
最新[2023]版：
大类|4 区医学
小类|4 区胃肠肝病学
第一作者：
第一作者机构：[1]From the *Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Sichuan, People's Republic of China[2]Department of Gastroenterological Surgery, West China Hospital, Sichuan University, Sichuan, People's Republic of China.
通讯作者：
通讯机构：[1]From the *Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Sichuan, People's Republic of China[*1]Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 1 Ke-Yuan-Si-Lu, Gao-Peng-Da-Dao, Chengdu, Sichuan, 610041, People's Republic of China
推荐引用方式(GB/T 7714)：
Liu Yong,Li Yang,Chen Ke-Ling,et al.Knockdown of Myeloid Differentiation Factor 88 Attenuates Lipopolysaccharide-Induced Inflammatory Response in Pancreatic Ductal Cells.[J].Pancreas.2016,45(5):755-60.doi:10.1097/MPA.0000000000000565.
APA：
Liu Yong,Li Yang,Chen Ke-Ling,Zhou Bin,Lv Zhao-Yin...&Li Yuan.(2016).Knockdown of Myeloid Differentiation Factor 88 Attenuates Lipopolysaccharide-Induced Inflammatory Response in Pancreatic Ductal Cells..Pancreas,45,(5)
MLA：
Liu Yong,et al."Knockdown of Myeloid Differentiation Factor 88 Attenuates Lipopolysaccharide-Induced Inflammatory Response in Pancreatic Ductal Cells.".Pancreas 45..5(2016):755-60
