The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling.
机构：[1]Department of Histology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.[2]Sichuan Cancer Hospital &四川省肿瘤医院[3]Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.四川省人民医院四川省肿瘤医院[3]Pathology Division, Shizuoka Cancer Center, Shizuoka, Japan.[4]Department of Translational Cancer Research, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan.[5]Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, India.[6]Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
The cold-shock protein Y-box-binding protein (YB)-1 regulates the expression of various chemokines and their receptors at the transcriptional level. Expression of the orphan chemokine CXCL14 is repressed by EGF induced signaling. The possible links between EGF-mediated YB-1 and CXCL14 as well as the functions of critical kinase pathways in the progression of prostate cancer have remained unexplored. Here we examined the correlation between YB-1 and CXCL14, and the ERK/AKT/mTOR pathways in prostate cancer. Knockdown of YB-1 decreased cyclinD1 expression with an upregulation of cleaved-PARP in human prostate cancer cells. EGF treatment upregulated phospho-YB-1 expression in a time-dependent manner, while treatment with an ERK inhibitor completely silenced its expression in prostate cancer cells. EGF treatment stimulates CyclinD1 and YB-1 phosphorylation in an ERK-dependent pathway. Positive and negative regulation of YB-1 and CXCL14 was observed after EGF treatment in prostate cancer cells, respectively. EGF rescues cell cycle and apoptosis via the AKT and ERK pathways. Furthermore, YB-1 silencing induces G1 arrest and apoptosis, while knockdown of CXCL14 facilitates cell growth and inhibits apoptosis in prostate cancer cells. YB-1 and CXCL14 were inversely correlated in prostate cancer cells and tissues. A significant association between poor overall survival and High YB-1 expression was observed in human prostate cancer patients. In conclusion, our data reveal the functional relationship between YB-1 and CXCL14 in EGF mediated ERK signaling, and YB-1 expression is a significant prognostic marker to predict prostate cancer.
基金：
This work was supported by Grants-in-Aid from the Ministry of Education, Culture,
Sports, Science and Technology of Japan; and the Scientific Research Funds project
of Science and Technology Department of Sichuan Province (grant no. 21YYJC1616
to Yang Liu), the Scientific Research Project of Sichuan Province (grant no. S2002 to
Yang Liu).
第一作者机构：[1]Department of Histology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
共同第一作者：
通讯作者：
通讯机构：[2]Sichuan Cancer Hospital &[5]Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, India.[6]Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.[*1]Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu,China[*2]Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
推荐引用方式(GB/T 7714)：
Wang Chen,Zhao Na,Sato Fuyuki,et al.The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling.[J].BIOENGINEERED.2021,12(2):9128-9139.doi:10.1080/21655979.2021.1993537.
APA：
Wang Chen,Zhao Na,Sato Fuyuki,Tanimoto Keiji,Okada Hiroyuki...&Bhawal Ujjal K.(2021).The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling..BIOENGINEERED,12,(2)
MLA：
Wang Chen,et al."The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling.".BIOENGINEERED 12..2(2021):9128-9139
