机构：[1]Institute of Neuroscience, Chongqing Medical University, Chongqing, China [2] Central Blood Bank, Sichuan Cancer Hospital and Research Institute, Chengdu, China四川省肿瘤医院
We previously reported that aquaporin 4 (AQP4) played a critical role in formation of brain edema and the altered expression of dystroglycan (DG) could relate with AQP4 expression after traumatic brain injury (TBI). However the mechanisms of this process remain unclear. DG was showed could act as a scaffold involved in adhesion-mediated signaling in ERK/MAPK pathway. We hypothesize that after scratch, extracellular alpha-DG and transmembrane beta-DG may act as the scaffold in scratch mechanical force activating ERK pathway which may regulate the expression of AQP4. Use ERK inhibitor and activator to confirm whether the expression of AQP4 is regulated by the activation of ERK pathway in scratched astrocytes. Use DG siRNA to confirm whether DG takes part in the process that the extracellular signal transduces into cell and activates the ERK pathway. The significant increase of AQP4 and DG expression induced by scratch could be abolished by blocking ERK signaling and enhanced by activating ERK signaling. Blockade of DG by siRNA led to no obvious effect of scratched-injury on the ERK signaling pathway. It demonstrated that DG may act as the scaffold in scratch mechanical force activating ERK pathway which can regulate the expression of AQP4 in astrocytes after scratch.
基金：
National Science Foundation of ChinaNational Natural Science Foundation of China [81500978, 81671312, 81000566]; National Science Foundation Project of Chong Qing [cstc2016jcyjA0229, cstc2017jcyjAX0414, cstc2015jcyja10018, cstc2011jjA10093]; Foundation of Chongqing Municipal Education Commission [KJ1600213]
第一作者机构：[1]Institute of Neuroscience, Chongqing Medical University, Chongqing, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Gaoli,Ma Peiying,Wan Shanshan,et al.Dystroglycan is involved in the activation of ERK pathway inducing the change of AQP4 expression in scratch-injured astrocytes[J].BRAIN RESEARCH.2019,1721:doi:10.1016/j.brainres.2019.146347.
APA：
Zhang, Gaoli,Ma, Peiying,Wan, Shanshan,Xu, Jin,Yang, Mei...&Liu, Hui.(2019).Dystroglycan is involved in the activation of ERK pathway inducing the change of AQP4 expression in scratch-injured astrocytes.BRAIN RESEARCH,1721,
MLA：
Zhang, Gaoli,et al."Dystroglycan is involved in the activation of ERK pathway inducing the change of AQP4 expression in scratch-injured astrocytes".BRAIN RESEARCH 1721.(2019)
