机构：[1]Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Cancer Hospital, Chengdu, China四川省肿瘤医院[2]Institute of Isotope, China Institute of Atomic Energy, Beijing, China
Ga-68 labeled FAPI is the current standard for FAPI-PET, but its batch activity is limited. [F-18]AlF-NOTA-FAPI-04 is a promising alternative combining the advantages of a chelator-based radiolabeling method with the unique properties of fluorine-18. The objective of this study was to develop a quick automatic method for synthesis of [F-18]AlF-NOTA-FAPI-04 using a AllinOne synthesis system, and perform PET imaging with [F-18]AlF-NOTA-FAPI-04 on patients. [F-18]AlF-NOTA-FAPI-04 was produced, and its quality control was conducted by HPLC equipped with a radioactive detector. [F-18]AlF-NOTA-FAPI-04 PET/CT imaging was performed in normal BALB/c mice (n = 3) and 4T1 breast cancer models (n = 3) to determine its biodistribution. Then [F-18]AlF-NOTA-FAPI-04 and F-18-fluorodeoxyglucose (FDG) PET/CT imaging were performed in an invasive ductal carcinoma patient (female, 54 years old). The synthesis time of [F-18]AlF-NOTA-FAPI-04 was about 25 min, and the radiochemical yield was 26.4 +/- 1.5% (attenuation correction, n = 10). The radiochemical purity was above 99.0% and was above 98.0% after 6 h. The product was colorless transparent solution with pH value of 7.0-7.5, and the specific activity was 49.41 +/- 3.19 GBq/mu mol. PET/CT imaging in mice showed that physiological uptake of [F-18]AlF-NOTA-FAPI-04 was mainly in the biliary system and bladder, and [F-18]AlF-NOTA-FAPI-04 highly concentrated in tumor xenografts. PET/CT imaging in the patient showed that [F-18]AlF-NOTA-FAPI-04 obtained high tumor background ratio (TBR) value of 8.44 in segment V and VI, while TBR value was 2.55 by F-18-FDG. [F-18]AlF-NOTA-FAPI-04 could be synthesized with high radiochemical yield and batch production by AllinOne module and show excellent diagnosis performance in cancer patients.
基金：
Sichuan Science and Technology Project [2019YJ0574, 2020YFS0421]
第一作者机构：[1]Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Cancer Hospital, Chengdu, China[2]Institute of Isotope, China Institute of Atomic Energy, Beijing, China
通讯作者：
推荐引用方式(GB/T 7714)：
Jiang Xiao,Wang Xiaoxiong,Shen Taipeng,et al.FAPI-04 PET/CT Using [F-18]AlF Labeling Strategy: Automatic Synthesis, Quality Control, and In Vivo Assessment in Patient[J].FRONTIERS IN ONCOLOGY.2021,11:doi:10.3389/fonc.2021.649148.
APA：
Jiang, Xiao,Wang, Xiaoxiong,Shen, Taipeng,Yao, Yutang,Chen, Meihua...&Cheng, Zhuzhong.(2021).FAPI-04 PET/CT Using [F-18]AlF Labeling Strategy: Automatic Synthesis, Quality Control, and In Vivo Assessment in Patient.FRONTIERS IN ONCOLOGY,11,
MLA：
Jiang, Xiao,et al."FAPI-04 PET/CT Using [F-18]AlF Labeling Strategy: Automatic Synthesis, Quality Control, and In Vivo Assessment in Patient".FRONTIERS IN ONCOLOGY 11.(2021)
