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The MRI-Visible Nanocomposite Facilitates the Delivery and Tracking of siRNA Loaded DC Vaccine in the Breast Cancer Model.

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机构: [1]Department of Radiology, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for ChildHealth and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory ofPediatrics, Chongqing, China, [2]Sichuan Key Laboratory of Medical Imaging and School of Medical Imaging, North SichuanMedical College, Nanchong, China, [3]National Engineering Research Center for Biomaterials Sichuan University, Chengdu, China, [4]Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China
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关键词: dendritic cell anticancer immunotherapy gene transfection nanocomposite magnetic resonance imaging

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Dendritic cell (DC) vaccines have recently been developed for the treatment of various cancers but often do not function as well as expected, primarily due to the highly complex in vivo immune environment. This proof-of-principle study aimed to test the feasibility of modulating the in vivo behaviors of DC vaccines (DCVs) by introducing siRNA-laden magnetic resonance (MR) imaging nanovectors into cells, while providing visible information on their homing to lymph nodes. The N-alkyl-PEI2k-LAC/SPIO nanocomposites were prepared and characterized, showing favorable properties of siRNA transfection and MRI labeling efficiency in DCs. Cell viability assays revealed no observable effects on the survival and phenotype of DCs if the concentration of the complex was within 8 μg Fe/ml. An orthotopic mouse model of breast cancer was developed. The DCVs transfected with IDO siRNA contained nanocomposites were adoptively transferred to start the treatment. MR imaging clearly visualized the homing of DCVs into lymph nodes. At the end of the treatment, DCVs presented significantly better tumor suppression than DCs or PBS (P < 0.05). Generally, the N-alkyl-PEI2k-LAC/SPIO nanocomposites represent a highly efficient MR imaging platform for siRNA transfection that is potentially useful for in vivo tracking of vaccine cells. Copyright © 2021 Wu, Zhu, Jin, Ai and Xu.

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出版当年[2020]版:
大类 | 3 区 医学
小类 | 3 区 肿瘤学
最新[2023]版:
大类 | 3 区 医学
小类 | 3 区 肿瘤学
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第一作者机构: [1]Department of Radiology, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for ChildHealth and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory ofPediatrics, Chongqing, China, [2]Sichuan Key Laboratory of Medical Imaging and School of Medical Imaging, North SichuanMedical College, Nanchong, China,
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