机构：[1]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Clinical Research Institute, Shanghai Jiao Tong University School of Medicine,Shanghai 200025, China[2]Shanghai Clinical Research Promotion andDevelopment Center, Shanghai Shenkang Hospital Development Center,Shanghai 200041, China[3]Key Laboratory of Cancer Prevention and Therapy,Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060,China[4]Department of Epidemiology, School of Public Health, China MedicalUniversity, Shenyang 110013, China[5]Cancer Epidemiology Program,University of Hawaii Cancer Center, 701 Ilalo Street, Honolulu, HI 96813, USA[6]Tongren Hospital, Shanghai Jiao Tong University School of Medicine,Shanghai 200336, China[7]Second Affiliated hospital of Chengdu MedicalCollege, China National Nuclear Corporation 416 Hospital, Chengdu 610051,Sichuan, China
Many cancer-associated single nucleotide polymorphisms (SNPs) are located in the genomic regions of long non-coding RNAs (lncRNAs). Mechanisms of these SNPs in connection to cancer risk are not fully understood.Association of SNP (rs140618127) in lncRNA LOC146880 with non-small cell lung cancer (NSCLC) was evaluated in a case-control study of 2707 individuals. The mechanism of the SNP's biologic influence was explored with in vitro and in vivo experiments, including plasmid transfection, siRNA knockdown, flow cytometry assessment, and assays of cell proliferation, migration, invasion, and colony formation.Association analysis showed that A allele of SNP rs140618127 was associated with low risk of NSCLC in the Chinese population. Lab experiments indicated that SNP rs140618127 contained a binding site for miR-539-5p and the binding between miR-539-5p and LOC146880 resulted in declined phosphorylation of an oncogene, ENO1. The reduced phosphorylation of ENO1 led to decreased phosphorylation of PI3K and Akt, which is further linked to the decline in cell proliferation and tumor progression.The study demonstrates that SNP rs140618127 in lncRNA loc146880 provides an alternate binding site for microRNA miR-539-5p which affects the phosphorylation of ENO1 and activation of the PI3K and Akt pathway.
基金：
National Natural Science Foundation of China (to BQ, Grant NO.81602929), the National Natural Science Foundation of China (to BQ, Grant NO.81973135), Tianjin Key Research and Development Program Science and Technology Support Key Projects (to MG, Grant No. 17YFZCSY00690), Startup Fund for Youngman Research at SJTU (to TF, 17X100040015), and Shanghai Jiao Tong University Medical and Industrial Cross Project (to TF, YG2017QN70).
第一作者机构：[1]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Clinical Research Institute, Shanghai Jiao Tong University School of Medicine,Shanghai 200025, China[2]Shanghai Clinical Research Promotion andDevelopment Center, Shanghai Shenkang Hospital Development Center,Shanghai 200041, China
共同第一作者：
通讯作者：
通讯机构：[1]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital/Clinical Research Institute, Shanghai Jiao Tong University School of Medicine,Shanghai 200025, China[7]Second Affiliated hospital of Chengdu MedicalCollege, China National Nuclear Corporation 416 Hospital, Chengdu 610051,Sichuan, China
推荐引用方式(GB/T 7714)：
Tienan Feng,Nannan Feng,Tengteng Zhu,et al.A SNP-mediated lncRNA (LOC146880) and microRNA (miR-539-5p) interaction and its potential impact on the NSCLC risk.[J].JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH.2020,39(1):doi:10.1186/s13046-020-01652-5.
APA：
Tienan Feng,Nannan Feng,Tengteng Zhu,Qiang Li,Qi Zhang...&Biyun Qian.(2020).A SNP-mediated lncRNA (LOC146880) and microRNA (miR-539-5p) interaction and its potential impact on the NSCLC risk..JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,39,(1)
MLA：
Tienan Feng,et al."A SNP-mediated lncRNA (LOC146880) and microRNA (miR-539-5p) interaction and its potential impact on the NSCLC risk.".JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 39..1(2020)
医学