机构：[1]State Key Laboratory of QualityResearch in Chinese Medicine,Macau Institute for Applied Researchin Medicine and Health, MacauUniversity of Science and Technology,Macau, China[2]Department ofNursing, The Affiliated Hospitalof Southwest Medical University,Sichuan, 646000, China[3]Departmentof Respiratory and Critical CareMedicine, Pulmonary and CriticalCare Medicine, The Affiliated Hospitalof Southwest Medical University,Sichuan, 646000, China[4]Departmentof Thoracic Surgery, The AffiliatedHospital of Southwest MedicalUniversity, Sichuan, 646000, China
The purpose of this study is to explore the potential biological roles of miR-101-5p in the progression of non-small-cell lung carcinoma (NSCLC).
The levels of miR-101-5p and chemokine (C-X-C motif) ligand 6 (CXCL6) in NSCLC tissues and cells were detected using the quantitative real-time PCR (qRT-PCR) assay. Proliferation, colony formation, migration and invasion assays were conducted using miR-101-5p-transfected NSCLC cells in vitro. The expression of CXCL6 was measured using immunofluorescence assay. Xenograft model and lung metastasis model were constructed to further reveal the precise roles of miR-101-5p in the lung metastasis and growth of NSCLC cells in vivo.
miR-101-5p was underregulated in NSCLC tissues when compared with that in the normal controls. The levels of miR-101-5p were lower in NSCLC cells (H1975, A549, HCC827 and H1650) than in non-tumorigenic human bronchial epithelial cells (BEAS-2B). Overregulation of miR-101-5p restrained the aggressiveness phenotypes of NSCLC cells in vitro. Furthermore, overregulation of miR-101-5p reduced the tumor growth and pulmonary metastasis of NSCLC cells in vivo. CXCL6 was the target gene of miR-101-5p in NSCLC. The mRNA levels of CXCL6 were negatively associated with the levels of miR-101-5p in NSCLC tissues. Finally, the rescue experiments suggested that the inhibitory role of miR-101-5p was mediated by regulating the expression of CXCL6 in NSCLC.
These findings indicated that overregulation of miR-101-5p restrained the progression of NSCLC cells by targeting CXCL6 and might function as a potential therapeutic target for NSCLC.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区医学
小类|3 区生物工程与应用微生物4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|3 区生物工程与应用微生物4 区肿瘤学
第一作者：
第一作者机构：[1]State Key Laboratory of QualityResearch in Chinese Medicine,Macau Institute for Applied Researchin Medicine and Health, MacauUniversity of Science and Technology,Macau, China[2]Department ofNursing, The Affiliated Hospitalof Southwest Medical University,Sichuan, 646000, China
通讯作者：
通讯机构：[2]Department ofNursing, The Affiliated Hospitalof Southwest Medical University,Sichuan, 646000, China[*1]Department of Nursing, The Affiliated Hospital of Southwest Medical University, No 25 Taiping Street, Luzhou, Sichuan, 646000, China
推荐引用方式(GB/T 7714)：
Chen Qi,Liu Dan,Hu Zhi,et al.miRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6.[J].OncoTargets and therapy.2019,12:835-848.doi:10.2147/OTT.S184235.
APA：
Chen Qi,Liu Dan,Hu Zhi,Luo Cheng&Zheng Si Lin.(2019).miRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6..OncoTargets and therapy,12,
MLA：
Chen Qi,et al."miRNA-101-5p inhibits the growth and aggressiveness of NSCLC cells through targeting CXCL6.".OncoTargets and therapy 12.(2019):835-848
