机构：[1]The First Department of General Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.[2]Institute of Hepatobiliary, Pancreas and Intestinal Disease, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.[3]Department of Pathology, The North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.[4]Department of Microbiology and Parasitology, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.
MicroRNAs (miRNAs) are small, non-coding RNAs that can function as oncogenes or tumor suppressors in human cancer. Abnormally expressed miR-224 was found to play a fundamental role in several types of cancer. The aim of this study was to investigate the prognostic and biological values of miR-224 in colorectal cancer (CRC).
Quantitative RT-PCR (qRT-PCR) was used to evaluate expression levels of miR-224. The postoperative survival rate was analyzed with Kaplan-Meier method. The roles of miR-224 in cell proliferation, migration and invasion were analyzed with pre-miR-224 transfected cells. In addition, the regulation of SMAD4 by miR-224 was evaluated by qRT-PCR, Western blotting and luciferase reporter assays.
In the present study, we demonstrated that miR-224 was significantly up-regulated in CRC tissue samples and associated with disease relapse and a relative poorer disease-free survival rate. Moreover, ectopic expression of miR-224 potently promoted tumor cell proliferation, migration and invasion in vitro. Furthermore, the over-expression of miR-224 in CRC cell lines decreased SMAD4 expression at the translational level and decreased SMAD4-driven luciferase-reporter activity.
Our data suggest that miR-224 could play an oncogenic role in the cellular processes of CRC and represent a novel biomarker for tumor relapse of CRC patients.
基金：
This study was supported by Scientific Research Fund of Sichuan Provincial
Education Department of China (CBY12-A-ZD16).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版：
大类|2 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]The First Department of General Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.[2]Institute of Hepatobiliary, Pancreas and Intestinal Disease, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.
共同第一作者：
通讯作者：
通讯机构：[1]The First Department of General Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.[2]Institute of Hepatobiliary, Pancreas and Intestinal Disease, North Sichuan Medical College, Nanchong, Sichuan, People’s Republic of China.
推荐引用方式(GB/T 7714)：
Zhang Guang-Jun,Zhou He,Xiao Hua-Xu,et al.Up-regulation of miR-224 promotes cancer cell proliferation and invasion and predicts relapse of colorectal cancer.[J].Cancer cell international.2013,13(1):104.doi:10.1186/1475-2867-13-104.
APA：
Zhang Guang-Jun,Zhou He,Xiao Hua-Xu,Li Yu&Zhou Tong.(2013).Up-regulation of miR-224 promotes cancer cell proliferation and invasion and predicts relapse of colorectal cancer..Cancer cell international,13,(1)
MLA：
Zhang Guang-Jun,et al."Up-regulation of miR-224 promotes cancer cell proliferation and invasion and predicts relapse of colorectal cancer.".Cancer cell international 13..1(2013):104
