机构：[1]Department of Gynecology and Obstetrics, the West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China[2]Department of Epidemiology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China[3]Department of Pathology, Cancer Institute and Hospital, Chinese Academy ofMedical Sciences, Peking Union Medical College, Beijing, China[4]Department of Pathology, University of Virginia, Charlottesville, VA[5]Global Cancer Initiative, Chestertown, MD[6]Global Coalition Against Cervical Cancer, Arlington, VA
We conducted a pilot study of whether nonpathologists could accurately diagnose cervical precancer in biopsies using only a basic light microscope, evaluating p16 immunohistochemistry (p16 IHC) of biopsies, and video-based training for both.
Using biopsies collected as part of a screening study conducted in rural China, we randomly selected 50 biopsies with a precancerous diagnosis of cervical intraepithelial neoplasia grade 2 (CIN2) or more severe (CIN2+) and 50 biopsies with diagnosis of CIN less severe than CIN2, and stained them for p16 using a commercial IHC kit. Twelve nonpathologists of varying educational backgrounds living in Beijing, China received video training and were assigned one of 4 sets of 25 CIN2+ and 25 CIN less severe than CIN2 for evaluation. A pathologist reviewed all 100 cases.
The mean sensitivity and specificity of the p16 IHC staining scored by the nonpathologists were 91.7% and 94.1%, respectively, compared to scoring by the pathologist. The readers and the pathologist agreed on p16 IHC scoring for 42 (84%) of the 50 slides of CIN less severe than CIN2 and 37 (74%) of the 50 CIN2+ slides. The mean sensitivity and specificity for consensus CIN2+ of p16 IHC as scored by the readers were 88% and 87%, respectively, versus an overall sensitivity and specificity by the pathologist of 96% and 92%, respectively.
We demonstrated that nonpathologists can accurately diagnose CIN2+ using p16 IHC alone.
基金：
The Bill & Melinda Gates Foundation and the NIH Fogarty International
Clinical Research Scholars Program (NIH; R24 TW007988) funded
this work.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区妇产科学
最新[2023]版：
大类|4 区医学
小类|4 区妇产科学
第一作者：
第一作者机构：[1]Department of Gynecology and Obstetrics, the West China Second University Hospital, Sichuan University, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China[2]Department of Epidemiology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China
通讯作者：
通讯机构：[5]Global Cancer Initiative, Chestertown, MD[6]Global Coalition Against Cervical Cancer, Arlington, VA[*1]Global Coalition Against Cervical Cancer, 3800 Fairfax Dr, Arlington, VA 22204.
推荐引用方式(GB/T 7714)：
Guang-Dong Liao,Le-Ni Kang,Wen Chen,et al.p16 Immunohistochemistry Interpretation by Nonpathologists as an Accurate Method for Diagnosing Cervical Precancer and Cancer.[J].Journal of lower genital tract disease.2015,19(3):207-11.doi:10.1097/LGT.0000000000000080.
APA：
Guang-Dong Liao,Le-Ni Kang,Wen Chen,Xun Zhang,Xiao-Yang Liu...&Philip E. Castle.(2015).p16 Immunohistochemistry Interpretation by Nonpathologists as an Accurate Method for Diagnosing Cervical Precancer and Cancer..Journal of lower genital tract disease,19,(3)
MLA：
Guang-Dong Liao,et al."p16 Immunohistochemistry Interpretation by Nonpathologists as an Accurate Method for Diagnosing Cervical Precancer and Cancer.".Journal of lower genital tract disease 19..3(2015):207-11
