机构：[a]Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA[b]State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China[c]Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[d]Biosystems and Biomaterials Division, National Institute of Standards & Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA[e]Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA[f]University of Maryland Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA[g]Mechanical Engineering Department, University of Maryland Baltimore County, Baltimore County, MD 21250, USA
Calcium phosphate cements (CPCs) are promising for dental and craniofacial repairs. The objectives of this study were to: (1) develop an injectable cell delivery system based on encapsulation of induced pluripotent stem cell-derived mesenchymal stem cells (iPSMSCs) in microbeads; (2) develop a novel tissue engineered construct by dispersing iPSMSC-microbeads in CPC to investigate bone regeneration in an animal model for the first time.
iPSMSCs were pre-osteoinduced for 2 weeks (OS-iPSMSCs), or transduced with bone morphogenetic protein-2 (BMP2-iPSMSCs). Cells were encapsulated in fast-degradable alginate microbeads. Microbeads were mixed with CPC paste and filled into cranial defects in nude rats. Four groups were tested: (1) CPC-microbeads without cells (CPC control); (2) CPC-microbeads-iPSMSCs (CPC-iPSMSCs); (3) CPC-microbeads-OS-iPSMSCs (CPC-OS-iPSMSCs); (4) CPC-microbeads-BMP2-iPSMSCs (CPC-BMP2-iPSMSCs).
Cells maintained good viability inside microbeads after injection. The microbeads were able to release the cells which had more than 10-fold increase in live cell density from 1 to 14 days. The cells exhibited up-regulation of osteogenic markers and deposition of minerals. In vivo, new bone area fraction (mean±SD; n=5) for CPC-iPSMSCs group was (22.5±7.6)%. New bone area fractions were (38.9±18.4)% and (44.7±22.8)% for CPC-OS-iPSMSCs group and CPC-BMP2-iPSMSCs group, respectively, 2-3 times the (15.6±11.2)% in CPC control at 12 weeks (p<0.05). Cell-CPC constructs accelerated scaffold resorption, with CPC-BMP2-iPSMSCs having remaining scaffold material that was 7-fold less than CPC control.
Novel injectable CPC-microbead-cell constructs promoted bone regeneration, with OS-iPSMSCs and BMP2-iPSMSCs having 2-3 fold the new bone of CPC control. Cell delivery accelerated scaffold resorption, with CPC-BMP2-iPSMSC having remaining scaffold material that was 7-fold less than CPC control. Therefore, CPC-microbead-iPSMSC is a promising injectable material for orthopedic, dental and craniofacial bone regenerations.
Published by Elsevier Ltd.
基金：
This study was supported by NIH
R01 DE14190 and R21 DE22625 (HX), National Science Foundation of China 81401794 (PW), 31100695 and 31328008 (LZ),
and University of Maryland School of Dentistry. This paper
was presented (PW) as an oral presentation at the 2015 IADR
meeting in Boston, and won the IADR Arthur R. Frechette
Prosthodontic Research Award
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|2 区工程技术
小类|1 区牙科与口腔外科3 区材料科学：生物材料
最新[2023]版：
大类|1 区医学
小类|1 区牙科与口腔外科1 区材料科学：生物材料
第一作者：
第一作者机构：[a]Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA[b]State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China
通讯作者：
通讯机构：[a]Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA[c]Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[e]Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA[f]University of Maryland Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA[g]Mechanical Engineering Department, University of Maryland Baltimore County, Baltimore County, MD 21250, USA[*1]Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA. Tel.: +1 410 706 7047.[*2]Department of Orthopaedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
推荐引用方式(GB/T 7714)：
Ping Wang,Yang Song,Michael D. Weir,et al.A self-setting iPSMSC-alginate-calcium phosphate paste for bone tissue engineering.[J].Dental materials : official publication of the Academy of Dental Materials.2016,32(2):252-63.doi:10.1016/j.dental.2015.11.019.
APA：
Ping Wang,Yang Song,Michael D. Weir,Jinyu Sun,Liang Zhao...&Hockin H.K. Xu.(2016).A self-setting iPSMSC-alginate-calcium phosphate paste for bone tissue engineering..Dental materials : official publication of the Academy of Dental Materials,32,(2)
MLA：
Ping Wang,et al."A self-setting iPSMSC-alginate-calcium phosphate paste for bone tissue engineering.".Dental materials : official publication of the Academy of Dental Materials 32..2(2016):252-63
工程技术