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Characterization of adenoviral transduction profile in prostate cancer cells and normal prostate tissue.

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机构： [1]Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China [2]Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts [3]Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts [4]Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, P.R. China [5]State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, P.R. China

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关键词： adeno virus gene delivery vector gene therapy prostate cancer prostate diseases

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Prostate diseases are common in males worldwide with high morbidity. Gene therapy is an attractive therapeutic strategy for prostate diseases, however, it is currently underdeveloped. As well known, adeno virus (Ad) is the most widely used gene therapy vector. The aims of this study are to explore transduction efficiency of Ad in prostate cancer cells and normal prostate tissue, thus further providing guidance for future prostate pathophysiological studies and therapeutic development of prostate diseases. We produced Ad expressing enhanced green fluorescence protein (EGFP), and characterized the transduction efficiency of Ad in both human and mouse prostate cancer cell lines in vitro, as well as prostate tumor xenograft, and wild-type mouse prostate tissue in vivo. Ad transduction efficiency was determined by EGFP fluorescence using microscopy and flow cytometry. Cell type-specific transduction was examined by immunofluorescence staining of cell markers. Our data showed that Ad efficiently transduced human and mouse prostate cancer cells in vitro in a dose dependent manner. Following intratumoral and intraprostate injection, Ad could efficiently transduce prostate tumor xenograft and the major prostatic cell types in vivo, respectively. Our findings suggest that Ad can efficiently transduce prostate tumor cells in vitro as well as xenograft and normal prostate tissue in vivo, and further indicate that Ad could be a potentially powerful toolbox for future gene therapy of prostate diseases. © 2017 Wiley Periodicals, Inc.

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出版当年[2017]版：

大类 | 2 区医学

小类 | 2 区泌尿学与肾脏学 3 区内分泌学与代谢

最新[2023]版：

大类 | 3 区医学

小类 | 3 区泌尿学与肾脏学 4 区内分泌学与代谢

第一作者：

第一作者机构： [1]Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China [2]Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts [3]Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts

通讯作者：

通讯机构： [1]Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China [2]Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts [3]Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts [5]State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, P.R. China [*1]Horae Gene Therapy Center, University of Massachusetts Medical School, 368 Plantation Street, AS6-2049, Worcester, MA 01605. [*2]Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Guoxue Xiang # 37, Chengdu, Sichuan 610041, P.R. China.

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