机构：[1]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China[2]Binzhou Medical University Hospital, Binzhou 256603, China[3]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China四川大学华西医院
Long non-coding RNAs (lncRNAs) are associated with the occurrence, development and prognoses of non-small cell lung cancer (NSCLC). In the present study, we investigated the functional mechanisms of the lncRNA XIST in two human NSCLC cell lines, A549 and NCI-H1299. In all the 5 NSCLC cell lines (NL9980, NCI-H1299, NCI-H460, SPC-A-1 and A549) tested, the expression levels of XIST were significantly elevated, as compared with those in normal human bronchial epithelial cell line BEAS-2B. In A549 and NCI-H1299 cells, knockdown of XIST by siRNA significantly inhibited the cell proliferation, migration and invasion, and promoted cell apoptosis. Furthermore, XIST knockdown elevated the expression of E-cadherin, and suppressed the expression of Bcl-2. Moreover, knockdown of XIST significantly suppressed the tumor growth in NSCLC A549 xenograft mouse model. Bioinformatic analysis and luciferase reporter assays revealed that XIST was negatively regulated by miR-449a. We further identified reciprocal repression between XIST and miR-449a, which eventually influenced the expression of Bcl-2: XIST functioned as a miRNA sponge of miR-449a, which was a negative regulator of Bcl-2. These data show that expression of the lncRNA XIST is associated with an increased growth rate and metastatic potential in NSCLC A549 and NCI-H1299 cells partially through miR-449a, and suggest that XIST may be a potential prognostic factor and therapeutic target for patients with NSCLC.
基金：
This study was supported by grants from the Ministry of Education Fund Priority to the Development of Instructions of Higher Leading Doctoral Degree Field (No 20131202130001, to Qing-hua ZHOU), the National Natural Science Foundation of China (No 81572288, to Qing-hua ZHOU; No 81302002, to Xue-bing LI), the Tianjin Natural Science Foundation (No 14JCQNJC12300, to Xue-bing LI) and the “New Century” Talent Training Project of Tianjin Medical University General Hospital (2014, to Xue-bing LI).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|2 区药学3 区化学综合
最新[2023]版：
大类|1 区医学
小类|1 区药学2 区化学：综合
第一作者：
第一作者机构：[1]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China[2]Binzhou Medical University Hospital, Binzhou 256603, China
通讯作者：
通讯机构：[1]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China[3]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China
推荐引用方式(GB/T 7714)：
Ya-long ZHANG,Xue-bing LI,Yan-xu HOU,et al.The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer.[J].Acta Pharmacologica Sinica.2017,38(3):371-381.doi:10.1038/aps.2016.133.
APA：
Ya-long ZHANG,Xue-bing LI,Yan-xu HOU,Nian-zhen FANG,Jia-cong YOU&Qing-hua ZHOU.(2017).The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer..Acta Pharmacologica Sinica,38,(3)
MLA：
Ya-long ZHANG,et al."The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer.".Acta Pharmacologica Sinica 38..3(2017):371-381
医学