机构：[1]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China[2]Binzhou Medical University Hospital, Binzhou 256603, China[3]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China
Long non-coding RNAs (lncRNAs) are associated with the occurrence, development and prognoses of non-small cell lung cancer (NSCLC). In the present study, we investigated the functional mechanisms of the lncRNA XIST in two human NSCLC cell lines, A549 and NCI-H1299. In all the 5 NSCLC cell lines (NL9980, NCI-H1299, NCI-H460, SPC-A-1 and A549) tested, the expression levels of XIST were significantly elevated, as compared with those in normal human bronchial epithelial cell line BEAS-2B. In A549 and NCI-H1299 cells, knockdown of XIST by siRNA significantly inhibited the cell proliferation, migration and invasion, and promoted cell apoptosis. Furthermore, XIST knockdown elevated the expression of E-cadherin, and suppressed the expression of Bcl-2. Moreover, knockdown of XIST significantly suppressed the tumor growth in NSCLC A549 xenograft mouse model. Bioinformatic analysis and luciferase reporter assays revealed that XIST was negatively regulated by miR-449a. We further identified reciprocal repression between XIST and miR-449a, which eventually influenced the expression of Bcl-2: XIST functioned as a miRNA sponge of miR-449a, which was a negative regulator of Bcl-2. These data show that expression of the lncRNA XIST is associated with an increased growth rate and metastatic potential in NSCLC A549 and NCI-H1299 cells partially through miR-449a, and suggest that XIST may be a potential prognostic factor and therapeutic target for patients with NSCLC.
基金：
Ministry of Education Fund Priority to the Development of Instructions of Higher Leading Doctoral Degree Field [20131202130001]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81572288, 81302002]; Tianjin Natural Science FoundationNatural Science Foundation of Tianjin [14JCQNJC12300]; "New Century" Talent Training Project of Tianjin Medical University General Hospital
语种：
外文
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区医学
小类|2 区药学3 区化学综合
最新[2023]版：
大类|1 区医学
小类|1 区药学2 区化学：综合
第一作者：
第一作者机构：[1]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China[2]Binzhou Medical University Hospital, Binzhou 256603, China
通讯作者：
通讯机构：[1]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China[3]Sichuan Lung Cancer Institute, Sichuan Lung Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China
推荐引用方式(GB/T 7714)：
Ya-long ZHANG,Xue-bing LI,Yan-xu HOU,et al.The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer.[J].Acta pharmacologica Sinica.2017,38(3):371-381.doi:10.1038/aps.2016.133.
APA：
Ya-long ZHANG,Xue-bing LI,Yan-xu HOU,Nian-zhen FANG,Jia-cong YOU&Qing-hua ZHOU.(2017).The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer..Acta pharmacologica Sinica,38,(3)
MLA：
Ya-long ZHANG,et al."The lncRNA XIST exhibits oncogenic properties via regulation of miR-449a and Bcl-2 in human non-small cell lung cancer.".Acta pharmacologica Sinica 38..3(2017):371-381
医学