机构：[1]College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu, P. R. China[2]Hospital of Medicine College, Northwest Minzu University, Lanzhou, Gansu, P. R. China[3]State Engineering Technology Institute for Karst Desertification Control, Guizhou Normal University, Guiyang, Guizhou, P. R. China[4]School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, P. R. China
The present study aimed to explore FSH receptor binding inhibitor (FRBI) effects on the levels of c-Myc, K-Ras and VEGF related to ovarian cancer, to evaluate the mRNA and protein levels of FSHR in the cumulus-oocyte complex (COCs). COCs were cultured for 24 h in the in vitro maturation (IVM) media replenished with 0, 10, 20, 30 and 40 μg/mL FRBI. Contents of c-Myc, K-Ras, VEGF, cAMP and IP3 in IVM media were detected with ELISA kits, respectively. The results indicated that the levels of FSHR protein and mRNA were determined with Western blotting. C-Myc contents of four FRBI + FSH-treated groups (COM groups) were reduced after IVM of COCs. C-Myc concentrations of COM-3 group was lower than the FSH group (p < .05). K-Ras and IP3 contents of COM-4 were decreased as compared to FSH group (p < .05). Expression levels of FSHR mRNAs and proteins in COM-4 group were smaller than that of FSH group. This study revealed that FRBI treatment could decrease c-Myc and K-Ras levels in the IVM medium fluids, and depress the FSHR levels of COCs. Expression levels of FSHR mRNAs and proteins of COM-4 group were significantly decreased. FRBI exerted its action via the signal pathway of IP3 and cAMP.
基金：
The Project of National Key Research and Development
Program of China in the 13th Five-year plan [2016 YFC
0502601], the Reproductive Biotechnology Innovation Team
of Animals of Colleges and Universities of Gansu Province
of China [2017 C-01] and National Natural Science
Foundation of the People’s Republic of China [31460684].
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|4 区农林科学
小类|4 区奶制品与动物科学4 区生物工程与应用微生物
最新[2023]版：
大类|3 区农林科学
小类|3 区奶制品与动物科学4 区生物工程与应用微生物
第一作者：
第一作者机构：[1]College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu, P. R. China
通讯作者：
通讯机构：[1]College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu, P. R. China[2]Hospital of Medicine College, Northwest Minzu University, Lanzhou, Gansu, P. R. China[*1]Hospital of Medicine College, Northwest Minzu University, No. 1, Xibeixincun Chengguan District, Lanzhou 730030, Gansu, P. R. China
推荐引用方式(GB/T 7714)：
Yang Juan,Gong Zhuandi,Shen Xiaoyun,et al.FSH receptor binding inhibitor depresses carcinogenesis of ovarian cancer via decreasing levels of K-Ras, c-Myc and FSHR.[J].Animal biotechnology.2021,32(1):84-91.doi:10.1080/10495398.2019.1656083.
APA：
Yang Juan,Gong Zhuandi,Shen Xiaoyun,Bai Shengju,Bai Xiaoqiang&Wei Suocheng.(2021).FSH receptor binding inhibitor depresses carcinogenesis of ovarian cancer via decreasing levels of K-Ras, c-Myc and FSHR..Animal biotechnology,32,(1)
MLA：
Yang Juan,et al."FSH receptor binding inhibitor depresses carcinogenesis of ovarian cancer via decreasing levels of K-Ras, c-Myc and FSHR.".Animal biotechnology 32..1(2021):84-91
