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Investigation of indoleamine 2,3-dioxygenase 1 expression in uveal melanoma.

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资源类型：
Pubmed体系：
作者：
Liang Chen[1,2] * ; Peng Lanya[3] ; Zeng Shaoxue[1,2] ; Zhao Qing[1,2] ; Tang Linqiao[4] ; Jiang Xiaoshuang[1] ; Zhang JunJun[1] ; Yan Naihong[1,2] ; Chen YingYing[1,2] ;
机构： [1]Research Laboratory of Ophthalmology and Vision Sciences, Torsten-Wiesel Research Institute of World Eye Organization, State Key Laboratory of Biotherapy, West China Hospital, SiChuan University, Chengdu, China [2]Department of Ophthalmology, West China Hospital, Sichuan University, Cheng Du, Sichuan, China [3]West China Hospital, Sichuan University, Cheng Du, Sichuan, China [4]Research Core Facility of West China Hospital, Sichuan University, China
出处：
DOI： 10.1016/j.exer.2019.01.005
ISSN：

关键词： Indoleamine 2 3-dioxygenase 1 Uveal melanoma Immune escape Bruch's membrane

摘要：
The purpose of this study was to investigate indoleamine 2,3-dioxygenase 1 (IDO1) expression and its implications in uveal melanoma (UM). Bioinformatics analysis was performed on microarray data (GSE22138 and GSE27831) from the Gene Expression Omnibus (GEO) database to evaluate IDO1 expression in mRNA level. Ninety-two cases in the database were divided into the IDO1-high group (46 cases) and IDO1-low group (46 cases). Paraffin embedded tumor sections from 27 patients with UM were studied by immunofluorescence. The mRNA results showed that IDO1 expression was inversely correlated with tumor thickness (9.93 ± 3.33 mm in IDO1-high group vs. 11.56 ± 2.38 mm in IDO1-low group) (p = 0.016) and metastatic rate (30.4% in IDO1-high group vs. 69.6% in IDO1-low group, p < 0.001). The IDO1-high group showed higher immune cell gene expression: CD3D (6.56 ± 1.0 vs. 5.46 ± 0.53, p < 0.0001), CD4 (4.72 ± 0.4 vs. 4.2 ± 0.42, p < 0.0001), and CD68 (6.17 ± 1.23 vs. 5.53 ± 0.77, p = 0.015). Further analysis showed that immune-suppressive T regulatory cell genes (CD3D, CD4, IL2RA and FOXP3) were expressed in 67.4% (31/46) cases in the IDO1-high group and 23.91% (11/46) cases in the IDO1-low group. In addition, IDO1 and interferon gamma (IFNG) mRNA expression were strongly correlated (r = 0.70, p < 0.0001). The correlation analysis of different immune checkpoints showed that IDO1 was positively correlated with CD274(PDL1), but not CTLA4 or PDCD1.The disease-free survival (DFS) in the IDO1-high/IFNG-high group was better than that of the IDO1-low/IFNG-low group. The IDO1 immunostaining result showed that 2 cases in 18 UMs with Bruch's membrane (BM) rupture and 7 out of 9 cases without BM rupture were scored high (Grade 2-3) (p = 0.001). Comparing the immune cells staining results between IDO1-high group and IDO1-low group, higher percentage of patients in the former group had high levels of T cells and macrophages infiltration, but only the difference in macrophage was statistically significant (CD68, 77.78% vs. 27.78%, p = 0.04). The analysis based on GEO data and the result from immunostaining study are consistent with each other. In conclusion, the expression of IDO1 is probably induced by IFNγ from infiltrated immune cells in UM. BM rupture is an important indicator of IDO1 expression level and distribution pattern. The complex role of IDO1 may limit its therapeutic effect in UM. Copyright © 2019 Elsevier Ltd. All rights reserved.

基金：
National Natural Science Foundation of China to Chen Liang (#81700850) and Xiaoshuang Jiang (#81500728).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类 | 2 区 医学
小类 | 2 区 眼科学
最新[2023]版：
大类 | 2 区 医学
小类 | 2 区 眼科学
第一作者：
第一作者机构： [1]Research Laboratory of Ophthalmology and Vision Sciences, Torsten-Wiesel Research Institute of World Eye Organization, State Key Laboratory of Biotherapy, West China Hospital, SiChuan University, Chengdu, China [2]Department of Ophthalmology, West China Hospital, Sichuan University, Cheng Du, Sichuan, China
通讯作者：
通讯机构： [1]Research Laboratory of Ophthalmology and Vision Sciences, Torsten-Wiesel Research Institute of World Eye Organization, State Key Laboratory of Biotherapy, West China Hospital, SiChuan University, Chengdu, China [2]Department of Ophthalmology, West China Hospital, Sichuan University, Cheng Du, Sichuan, China [*1]#1 keyuan 4 road, Gao Xin district, Cheng Du, Sichuan province, 610041, China
推荐引用方式(GB/T 7714)：
Liang Chen,Peng Lanya,Zeng Shaoxue,et al.Investigation of indoleamine 2,3-dioxygenase 1 expression in uveal melanoma.[J].Experimental eye research.2019,181:112-119.doi:10.1016/j.exer.2019.01.005.
APA：
Liang Chen,Peng Lanya,Zeng Shaoxue,Zhao Qing,Tang Linqiao...&Chen YingYing.(2019).Investigation of indoleamine 2,3-dioxygenase 1 expression in uveal melanoma..Experimental eye research,181,
MLA：
Liang Chen,et al."Investigation of indoleamine 2,3-dioxygenase 1 expression in uveal melanoma.".Experimental eye research 181.(2019):112-119

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