机构：[1]Department of Urology, Institute of Urology[2]Core Facility, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China四川大学华西医院
Ribosomal protein S15A (RPS15A), a member of the ribosomal protein gene family, was demonstrated to be closely associated with tumorigenesis in multiple human malignancies. Nevertheless, the role of RPS15A in the progression of renal cell carcinoma (RCC) remains unknown. In the present study, by comparing the publicly available data from RCC tissues and reverse transcription‑quantitative polymerase chain reaction results, it was identified that RPS15A was upregulated in RCC tissues and cell lines (P<0.001). Notably, knockdown of RPS15A suppressed 786‑O cell proliferation (P<0.001) and promoted its apoptosis/necrotic (P=0.0001) in vitro. Additionally, tumour formation and growth of transfected 786‑O cells were observed to be restrained in a mouse model (P<0.05). Subsequent to analysing the microarray data, 747 genes were differentially expressed in the RPS15A‑knockdown 786‑O cells. The enriched canonical pathways, diseases and functions of differentially expressed genes, and the interactive network of RPS15A in RCC were successfully constructed by ingenuity pathway analysis. Overall, the present results provided a preliminary experimental basis for RPS15A as a novel oncogene and potential therapeutic target in RCC.
基金：
The Science and Technology Foundation of Sichuan Province (grant no. 2014JY0183 to Yiping Lu and grant no. 2017SZ0123 to Zhihong Liu) and 1.3.5 Project for Disciplines of Excellence, West China Hospital, Sichuan University (Chengdu, China).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|4 区肿瘤学4 区医学：研究与实验
最新[2023]版：
大类|3 区医学
小类|4 区医学：研究与实验4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Urology, Institute of Urology
共同第一作者：
通讯作者：
通讯机构：[1]Department of Urology, Institute of Urology[*1]Department of Urology, Institute of Urology, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, Sichuan 610041, P.R. China
推荐引用方式(GB/T 7714)：
Liang Jiayu,Liu Zhihong,Zou Zijun,et al.Knockdown of ribosomal protein S15A inhibits human kidney cancer cell growth in vitro and in vivo.[J].Molecular medicine reports.2019,19(2):1117-1127.doi:10.3892/mmr.2018.9751.
APA：
Liang Jiayu,Liu Zhihong,Zou Zijun,Wang Xiangxiu,Tang Yongquan...&Lu Yiping.(2019).Knockdown of ribosomal protein S15A inhibits human kidney cancer cell growth in vitro and in vivo..Molecular medicine reports,19,(2)
MLA：
Liang Jiayu,et al."Knockdown of ribosomal protein S15A inhibits human kidney cancer cell growth in vitro and in vivo.".Molecular medicine reports 19..2(2019):1117-1127
