Long non-coding RNA NEAT1 regulates epithelial membrane protein 2 expression to repress nasopharyngeal carcinoma migration and irradiation-resistance through miR-101-3p as a competing endogenous RNA mechanism
机构：[1]Department of Urology and State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, P.R. China四川大学华西医院[2]Department of Radiation Oncology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, P.R. China四川省人民医院四川省肿瘤医院[3]School of Pharmacy, Chengdu Medical College, Chengdu 611137, P.R. China
The altered expression of long non-coding RNAs (lncRNAs) is often related to carcinogenesis, metastasis and resistance to radiation or chemotherapy. In the current study, cDNA microarray analysis found that NEAT1 expression was reduced in nasopharyngeal carcinoma (NPC) patients and that it regulated NPC progression. However, the detailed mechanisms of NEAT1 in NPC were unclear. NEAT1 repressed NPC cell growth, invasion and radiation resistance in vitro and tumor metastasis in vivo. In addition, the results of an approach integrating bioinformatics, luciferase reporter assays and RNA immunoprecipitation indicated that NEAT1 antagonized miR-101-3p through a competing endogenous RNA (ceRNA) mechanism and that the interaction between NEAT1 and EMP2 was miR-101-3p dependent. Our results showed a novel connection of NEAT1, miR-101-3p and EMP2 in NPC migration and radiation resistance.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81672553]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2016M602696]; Sichuan Youth Science and Technology Foundation [2016JQ0060]; Sichuan UniversitySichuan University [2015SCU04A13]
第一作者机构：[1]Department of Urology and State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, P.R. China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Yujia Wang,Chunting Wang,Can Chen,et al.Long non-coding RNA NEAT1 regulates epithelial membrane protein 2 expression to repress nasopharyngeal carcinoma migration and irradiation-resistance through miR-101-3p as a competing endogenous RNA mechanism[J].ONCOTARGET.2017,8(41):70156-70171.doi:10.18632/oncotarget.19596.
APA：
Yujia Wang,Chunting Wang,Can Chen,Fengbo Wu,Pengfei Shen...&Xiang Li.(2017).Long non-coding RNA NEAT1 regulates epithelial membrane protein 2 expression to repress nasopharyngeal carcinoma migration and irradiation-resistance through miR-101-3p as a competing endogenous RNA mechanism.ONCOTARGET,8,(41)
MLA：
Yujia Wang,et al."Long non-coding RNA NEAT1 regulates epithelial membrane protein 2 expression to repress nasopharyngeal carcinoma migration and irradiation-resistance through miR-101-3p as a competing endogenous RNA mechanism".ONCOTARGET 8..41(2017):70156-70171
