Objection: The aim of this study was to evaluate the effects of lncRNA GHET1 in developing of Gastric Cancer. Methods: Collecting the 20 gastric cancer patients and evaluated the pathological of adjacent and tumor tissues by HE stating. We analyzed the protein expression of Numb in gastric cancer (GC) tissues by using IHC and the gene expression of Numb and GHET1 in adjacent and GC tissues by RT-PCR. The AGS cells were divided into 3 groups: Control (Co), NC and shRNA groups. Measuring the cell proliferation, apoptosis, cell cycle, invasion and migration abilities by MTT, flow cytometry, transwell and wound healing assays. We analyzed the relative signaling pathway by WB assay. Results: In the clinical analyzing, compared with adjacent tissues, the pathological was significantly changed in tumor tissues, the GHET1 gene and protein expressions were significantly increased in the GC tissues. In the cell experiment, down-regulation of GHET1 had suppressed the cell proliferation, invasion and migration activities and enhanced the cell apoptosis and G1 phase. We found that knockdown of GHET1 dramatically increased E-cadherin, while reducing fibronectin and vimentin. Conclusion: lncRNA GHET1 promoted AGS cells activations, and the results were shown that GHET1 dysregulation might be involved in the occurrence and development of gastric cancer. These results suggested that GHET1 might be a molecular marker for the progression of gastric cancer and a molecular target for targeted therapy. (C) 2017 Published by Elsevier Masson SAS.