Recent studies reported that long non-coding RNA NEAT1 was dysregulated expression in some cancers and also closely related with malignant phenotype of lymphatic metastasis and TNM stage. However, the detailed effect mechanism of NEAT1 still remains mysterious. NEAT1 and ZEB2 were up-regulated in CRC, meanwhile, miR-662 presented down-regulated in CRC tissues, compared with adjacent normal intestinal mucosal tissues (all p<0.05); Correlation analysis showed that NEAT1 expression was negatively with miR-662 in CRC tissues, miR-662 expression was negatively with ZEB2 and NEAT1 was positively with ZEB2 (all p<0.01). Either knowing-down NEAT1 or over-expressing miR-662 inhibited invasion and migration of colon cancer cells. Both knowing-down NEAT1 and over-expressing miR-662 could suppress expression of EMT relative molecules of ZEB2, Fibonectin and SNAIL. Luciferase report assay, RIP test and RNA Pull-Down confirmed that NEAT1 could directly sponge miR-662 and miR-662 could directly target ZEB2. Consequently, NEAT1 can directly sponge miR-662 which can directly target ZEB2, to promote invasion and migration of colon cancer cells. NEAT1-miR-662-ZEB2 axis is formed to regulate invasion and metastasis of CRC.