机构：[1]Department of Thoracic Surgery, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center,School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041外科中心胸外科中心四川省人民医院四川省肿瘤医院胸外科[2]Department of Thoracic Surgery, First Affiliated Hospital of Kunming Medical University, Kunming,Yunnan 650000昆明医科大学附属第一医院[3]Laboratory of Molecular Diagnosis of Cancer, West China Hospital, Sichuan University四川大学华西医院[4]Department of Chemotherapy, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine,University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, P.R. China四川省肿瘤医院
Period2 (Per2) is a key circadian clock gene, and its deregulation contributes to tumour development, including breast cancer. However, the biological function and clinicopathological significance of Per2 in non-small cell lung cancer (NSCLC) remain unclear. The present study aimed to explore the role of Per2 and its relative clinical significance in NSCLC. To analyse Per2 expression in NSCLC specimens, reverse transcription-quantitative polymerase chain reaction was performed, and the results indicated that Per2 expression was markedly downregulated in 83.87% (26/31) of NSCLC samples compared with their adjacent matched tissues. Increased Per2 expression was associated with increased differentiation (P<0.01) and reduced lymph node metastasis (P<0.0001). Functional studies identified that enhancing Per2 expression in A549 cells by lentivirus transduction not only significantly suppressed cell growth, migration and invasion (P<0.05) but also inhibited NSCLC growth and metastasis in vivo. Animal studies and histopathological analysis identified that Per2 expression in A549 cells not only markedly increased expression of tumour anti-oncogenes Bax, P53 and P21 but also inhibited expression of pro-oncogenes vascular endothelial growth factor, CD44 and c-Myc. These results indicate that the loss of Per2 is one of the factors underlying tumourigenesis in NSCLC, and it may function as a novel molecular target for NSCLC.
基金：
Scientific Research Foundation of the Technology Department of Sichuan Province, China [2017SZ0013, 2018SZ0153]
第一作者机构：[1]Department of Thoracic Surgery, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center,School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041
共同第一作者：
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center,School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610041[4]Department of Chemotherapy, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine,University of Electronic Science and Technology of China, Chengdu, Sichuan 610041, P.R. China[*1]Department of Thoracic Surgery, Sichuan Cancer Hospital, 55 Renmin South Road, Chengdu, Sichuan 610041, P.R. China[*2]Department of Chemotherapy, Sichuan Cancer Hospital, 55 Renmin South Road, Chengdu, Sichuan 610041, P.R. China
推荐引用方式(GB/T 7714)：
Xiang Run,Cui Yue,Wang Yanping,et al.Circadian clock gene Per2 downregulation in non-small cell lung cancer is associated with tumour progression and metastasis[J].ONCOLOGY REPORTS.2018,40(5):3040-3048.doi:10.3892/or.2018.6704.
APA：
Xiang, Run,Cui, Yue,Wang, Yanping,Xie, Tianpeng,Yang, Xiaojun...&Li, Qiang.(2018).Circadian clock gene Per2 downregulation in non-small cell lung cancer is associated with tumour progression and metastasis.ONCOLOGY REPORTS,40,(5)
MLA：
Xiang, Run,et al."Circadian clock gene Per2 downregulation in non-small cell lung cancer is associated with tumour progression and metastasis".ONCOLOGY REPORTS 40..5(2018):3040-3048
