Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/beta-catenin signaling
机构：[1]Institute of Life Sciences, Wenzhou University, Wenzhou, China[2]Sichuan Provincial Medical Center of Mental Health & Sichuan Provincial People's Hospital, Sichuan, China四川省人民医院[3]School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China[4]Bioengineering College, Chongqing University, Chong qing, China[5]The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
Lung cancer (LC) has the highest mortality of all tumors. Non-small cell lung cancer (NSCLC) accounts for about 80% of all LC. Acanthopanax senticosus polysaccharide (ASPS) is extracted from the root of Acanthopanax senticosus (AS). Herein, we examined the effect and molecular mechanism of ASPS on NSCLC. The proliferation, invasion and migration of NCI-H520 cells were detected by cell counting kit-8 (CCK-8), transwell assay and wound healing assay, respectively. The epithelial-mesenchymal transition (EMT) and Wnt/beta-catenin pathway-related factors were evaluated using quantitative real-time PCR (qRT-PCR) and western blot assay. Our results showed that ASPS significantly decreased the proliferation of cells at 24 and 48 h. Moreover, ASPS markedly repressed the invasion and migration capacities of cells in a concentration-dependent manner. Besides, ASPS obviously downregulated the levels of matrix metalloproteinase-2 (MMP-2), MMP-9, fibronectin 1 (FN1), vimentin, wnt3a, phosphorylated-glycogen synthase kinase 3 beta (p-GSK3 beta) and cyclin D1, whereas E-cadherin level was upregulated. The level of GSK3 beta was not changed within the different groups. ASPS conspicuously inhibited the abilities of proliferation and metastasis in human non-small cell lung cancer cell line NCI-H520 possibly by suppressing Wnt/beta-catenin pathway mediated-EMT.
基金：
Wenzhou Science & Technology Innovation Project in Water Pollution Control Manage [W20170010]; Zhejiang Pharmaceutical Top Priority Academic Open Fund [201720]
第一作者机构：[1]Institute of Life Sciences, Wenzhou University, Wenzhou, China
通讯作者：
推荐引用方式(GB/T 7714)：
Sun D.,Chen J.,Hu H.,et al.Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/beta-catenin signaling[J].NEOPLASMA.2019,66(4):555-563.doi:10.4149/neo_2018_180913N689.
APA：
Sun, D.,Chen, J.,Hu, H.,Lin, S.,Jin, L....&Zhang, C..(2019).Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/beta-catenin signaling.NEOPLASMA,66,(4)
MLA：
Sun, D.,et al."Acanthopanax senticosus polysaccharide suppressing proliferation and metastasis of the human non-small cell lung cancer NCI-H520 cells is associated with Wnt/beta-catenin signaling".NEOPLASMA 66..4(2019):555-563
