机构：[1]Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China[2]Institute of Neuronscience, Kunming Medical University, Kunming 650031, China[3]Institute of Neurological Diseases,Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院
The recovery of motor function in rats is inhibited following contusion spinal cord injury (cSCI). However, the mechanism of tumour necrosis factor α (TNF-α) in motor function after cSCI associated with peroxiredoxin 6 (PRDX6) remains unknown. We randomly divided rats into four groups: sham, cSCI, vector and lentivirus mediating TNF-α RNA interference (TNF-α-RNAi-LV) group. The Basso, Beattie, Bresnahan (BBB) scale was used to evaluate motor function. Real-time quantitative PCR (qRT-PCR) and western blotting were used to detect the expression of TNF-α and PRDX6, which were located in neurons using immunohistochemistry (IHC) and immunofluorescence. Subsequently, lentiviral-mediated TNF-α was used to determine the role of TNF-αand the relationship of PRDX6 and TNF-α in cSCI. After cSCI, the motor capability of hind limbs disappeared and was followed by recovery of function. IHC analysis indicated that TNF-α and PRDX6 were primarily located in spinal cord neurons. TNF-α interference significantly improved neural behaviour and increased expression of PRDX6. Our study suggests that inhibition of TNF-α can promote the recovery of motor function. The underlying mechanism of TNF-α-promoted motor function may be connected with the up-regulation of PRDX6. This provides a new strategy or target for the clinical treatment of SCI in future.
基金：
This work was supported by the National undergraduate innovation training project
(201313705005) and the Provincial undergraduate innovation training project
(201313705029, 201313705030)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]Center for Experimental Technology of Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[2]Institute of Neuronscience, Kunming Medical University, Kunming 650031, China[3]Institute of Neurological Diseases,Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
推荐引用方式(GB/T 7714)：
Zhang Xiao,Shi Lan-lan,Gao Xia,et al.Lentivirus-mediated inhibition of tumour necrosis factor-α improves motor function associated with PRDX6 in spinal cord contusion rats.[J].Scientific reports.2015,5:8486.doi:10.1038/srep08486.
APA：
Zhang Xiao,Shi Lan-lan,Gao Xia,Jiang Di,Zhong Zhan-qiong...&Wang Ting-hua.(2015).Lentivirus-mediated inhibition of tumour necrosis factor-α improves motor function associated with PRDX6 in spinal cord contusion rats..Scientific reports,5,
MLA：
Zhang Xiao,et al."Lentivirus-mediated inhibition of tumour necrosis factor-α improves motor function associated with PRDX6 in spinal cord contusion rats.".Scientific reports 5.(2015):8486
