Surface-enhanced Raman scattering (SERS) nanotags as an ultrasensitive nanoprobe is becoming popular for the detection of biomarkers. Herein, antibody-conjugated gold nanoparticles (AuNPs) were used to target LMP2A in an LMP2A-infected CNE2 cell line. SERS maps showed that the LMP2A was distributed around the cell, which was consistent with the results of immunofl uorescence staining in the previous report. This location could be due to the specific binding of the bioconjugated nanotags to the receptors on the cell surface. However, the CNE2 cell line without LMP2A-infected showed no detectable signal at 1044 cm(-1). The results demonstrated the potential feasibility of AuNPs nanotags as highly sensitive probes conjugated at the subcellular level for detection and localization of cancer markers in nasopharyngeal carcinoma (NPC).
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31200629]; Science and Technology Planning Project of Guangdong Province [2015A050502006]; Shenzhen Science and Technology Innovation Committee [JCYJ20150417094158018, GJHZ20150316112419786, JCYJ20150417094158016]
通讯机构：[1]Shenzhen Inst Informat Technol, Sch Mech & Elect Engn, Shenzhen 518172, Peoples R China;[2]Sun Yat Sen Univ, Dept Expt Res, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China,Canc Ctr, Guangzhou 510060, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Li J. -H.,Du Y.,Feng G. -K.,et al.Sers Imaging of Nasopharyngeal Carcinoma Markers Using an Antibody-Conjugated Gold Nanoparticles Probe[J].JOURNAL OF APPLIED SPECTROSCOPY.2017,84(5):790-795.doi:10.1007/s10812-017-0546-8.
APA：
Li, J. -H.,Du, Y.,Feng, G. -K.,Du, Y. -B.,Zhou, Y. -Q.&Zeng, M. -S..(2017).Sers Imaging of Nasopharyngeal Carcinoma Markers Using an Antibody-Conjugated Gold Nanoparticles Probe.JOURNAL OF APPLIED SPECTROSCOPY,84,(5)
MLA：
Li, J. -H.,et al."Sers Imaging of Nasopharyngeal Carcinoma Markers Using an Antibody-Conjugated Gold Nanoparticles Probe".JOURNAL OF APPLIED SPECTROSCOPY 84..5(2017):790-795
