机构：[1]State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China其他部门华南肿瘤学国家重点实验室中山大学肿瘤防治中心[2]Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China中山大学肿瘤防治中心[3]Department of Colorectal Surgery, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China临床科室结直肠科中山大学肿瘤防治中心[4]Guangdong Lung Cancer Institute, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China肿瘤治疗中心研究所肺肿瘤科广东省肺癌研究所广东省人民医院[5]Department of Hematology/Oncology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510000, China[6]Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
Long noncoding RNAs (lncRNAs) have been shown to play critical roles in the biology of various cancers. However, their expression patterns and biological functions in human colorectal cancer (CRC) remain largely unknown. The aim of this study was to explore lncRNA profiles in CRC and investigate key lncRNAs involved in CRC tumorigenesis and progression. The microarray data of six CRC and matched non-cancerous tissues revealed distinct lncRNA profiles, including 899 upregulated and 1,646 downregulated lncRNAs (p < 0.05, fold change > 2.0). Furthermore, we found that the lncRNA BC032913 was generally underexpressed in 115 CRC samples compared with normal tissues. Reduced BC032913 levels were significantly associated with an advanced tumor, lymph nodes, distant metastasis (TNM) stage and a higher risk of lymph node and distant metastases. BC032913 downregulation indicated poor overall survival in CRC patients. Moreover, BC032913 enhanced the mRNA and protein expression of TIMP3 and inhibited Wnt/beta-catenin pathway activity, thus suppressing CRC metastasis in vitro and in vivo. Collectively, the obtained data show that BC032913 plays an inhibitory role in CRC aggression by upregulating TIMP3, followed by inactivation of the Wnt/b-catenin pathway. Our findings indicate that the novel lncRNA BC032913 could serve as a novel prognostic marker and effective therapeutic target for CRC.
基金：
National Natural Science Foundation of China [81272513, 81272638]; National High Technology Research and Development Program of China (863 Program) [2012AA02A204]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|2 区医学
小类|2 区医学：研究与实验
最新[2023]版：
大类|2 区医学
小类|2 区医学：研究与实验
第一作者：
第一作者机构：[1]State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China[2]Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China[4]Guangdong Lung Cancer Institute, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China
共同第一作者：
通讯作者：
通讯机构：[1]State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China[2]Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China[*1]State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, No. 651 Dongfeng East Road, Guangzhou 510060, China.
推荐引用方式(GB/T 7714)：
Lin Jiaxin,Tan Xin,Qiu Lin,et al.Long Noncoding RNA BC032913 as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3[J].MOLECULAR THERAPY-NUCLEIC ACIDS.2017,8:469-481.doi:10.1016/j.omtn.2017.07.009.
APA：
Lin, Jiaxin,Tan, Xin,Qiu, Lin,Huang, Long,Zhou, Yi...&Huang, Wenlin.(2017).Long Noncoding RNA BC032913 as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3.MOLECULAR THERAPY-NUCLEIC ACIDS,8,
MLA：
Lin, Jiaxin,et al."Long Noncoding RNA BC032913 as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3".MOLECULAR THERAPY-NUCLEIC ACIDS 8.(2017):469-481
医学