Circulating miR-221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression
机构：[1]Departments of Medical Oncology,Cancer Center, Sun Yat-sen University, Guangzhou 510060[2]Departments of Research ,Cancer Center, Sun Yat-sen University, Guangzhou 510060[3]Departments of Pathology, Cancer Center, Sun Yat-sen University, Guangzhou 510060,[4]State Key Laboratory of Oncology in Southern China,其他部门华南肿瘤学国家重点实验室中山大学肿瘤防治中心[5]Department of Medical Oncology, HuNan Cancer Hospital, 410013, ChangSha, China,[6]Cancer Research Center, Guangdong Medical College, 524023, Zhanjiang, China,[7]Department of Medical Oncology, The First Affiliated Hospital, Sun Yat-sen University,其他科室肿瘤科中山大学附属第一医院[8]GuangDong General Hospital, Guangzhou 510080, China广东省人民医院
Background and Aim: Circulating miRNAs exist in serum and plasma and they can be used as a potential noninvasive molecular marker for colorectal cancer (CRC) diagnosis. The present study was to test the availability of direct amplification of miRNAs from plasma without RNA extraction, and to evaluate its clinical application value in CRC. Methods: Plasma miR-21, miR-221 and miR-222 levels were determined in 103 CRC patients and 37 healthy normal controls by quantitative reverse transcription-polymerase chain reaction. Immunohistochemical staining for p53, carcinoembryonic antigen (CEA), estrogen receptor (ER) and progesterone receptor (PR) was carried out in the same CRC patient cohort. The correlation between miR-221 levels and protein levels of p53, CEA, ER and PR, clinicopathological features or overall survival was analyzed. Results: A standard curve shows a good linearity between the log of sample input and C-T values over three orders of magnitude of plasma miR-21, miR-221 and miR-222. ROC curve analysis reveals that the plasma levels of miR-221 is a potential biomarker for differentiating CRC patients from controls. Kaplan-Meier curve assessment shows that the elevated plasma miR-221 level is a significant prognostic factor for poor overall survival in CRC patients. The immunohistochemistry analysis demonstrates a significant correlation between plasma miR-221 level and p53 expression. Conclusions: The direct amplification of plasma miR-221 can be used as a potential noninvasive molecular marker for diagnosis and prognosis of CRC and is correlated with p53 expression.
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外文
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出版当年[2010]版：
大类|3 区医学
小类|4 区胃肠肝病学
最新[2023]版：
大类|3 区医学
小类|3 区胃肠肝病学
第一作者：
第一作者机构：[1]Departments of Medical Oncology,Cancer Center, Sun Yat-sen University, Guangzhou 510060[4]State Key Laboratory of Oncology in Southern China,[5]Department of Medical Oncology, HuNan Cancer Hospital, 410013, ChangSha, China,
共同第一作者：
通讯作者：
通讯机构：[1]Departments of Medical Oncology,Cancer Center, Sun Yat-sen University, Guangzhou 510060[4]State Key Laboratory of Oncology in Southern China,[*1]Department of Medical Oncology, Cancer Center, Sun Yat-sen University, 651 DongFeng Road, Guangzhou 510060, China.
推荐引用方式(GB/T 7714)：
Pu Xing-xiang,Huang Guo-liang,Guo Hong-qiang,et al.Circulating miR-221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression[J].JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY.2010,25(10):1674-1680.doi:10.1111/j.1440-1746.2010.06417.x.
APA：
Pu, Xing-xiang,Huang, Guo-liang,Guo, Hong-qiang,Guo, Cheng-cheng,Li, Haoran...&Lin, Tong-yu.(2010).Circulating miR-221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression.JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,25,(10)
MLA：
Pu, Xing-xiang,et al."Circulating miR-221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression".JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 25..10(2010):1674-1680
