To address the rational use of medicines in healthcare institutions, the Chinese government enacted legislation for pharmacists in 2021, emphasizing the importance of pharmacists in medical services. The allocation and equity of pharmacist human resources in healthcare institutions directly affect people's accessibility to pharmaceutical services. The study aims to analyze the supply and demand of pharmacist human resources in Chinese healthcare institutions and evaluate the equity of their distribution to provide a reference for the rational management of pharmacist resources.This was a time-series study that was conducted using data obtained from Health and Health Statistical Yearbook that is performed every year by the National Health Commission of the People's Republic of China. The study included 31 provinces, municipalities, and autonomous regions and 34,354 medical institutions in mainland China, which were classified into different economic regions based on their level of economic development. The study collected healthcare technicians and pharmacist data from national healthcare institutions between 2016 and 2020. The study evaluated the level of pharmacist human resource allocation using the health resource density index. The study explored the equity of pharmacist human resource allocation from two aspects: population and geographic area, using the Gini coefficient and Theil index.The average annual growth rates of healthcare technical staff and pharmacists in Chinese medical institutions from 2016 to 2020 were 5.67% and 3.03%, respectively. The Gini coefficients of healthcare personnel resources based on population distribution were all below 0.119, while those based on geographical area were all above 0.655. The total Theil indexes of pharmacist human resources based on population distribution were all below 0.010, while those based on geographical area were all above 0.511. In addition, the contribution rates of the within-group Theil indexes of pharmacist human resources based on population and geographical area distributions to the total Theil index were all greater than or equal to 50%.The allocation level of pharmacist human resources in China continues to improve, but there are differences in different economic development areas. Currently, allocation based on population shows equity, but allocation based on geographic area shows inequity. In the future, more attention should be paid to the geographic accessibility of pharmacist human resources, and resource allocation should consider both the population served and geographic area.© 2024. The Author(s).