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Pseudolaric acid B suppresses NSCLC progression through the ROS/AMPK/mTOR/autophagy signalling pathway

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资源类型：
Pubmed体系：
作者：
Luo Dan[1,2,3] * ; He Fang[2,3] * ; Liu Jingyun[2,3] * ; Dong Xueting[2,3] * ; Fang Mengying[2,3] ; Liang Yuling[2,3] ; Chen Mengqin[2,3] ; Gui Xuemei[2,3] ; Wang Wenjun[2,3] ; Zeng Li[1] ; Fan Xianming[2,3,*1] ; Wu Qibiao[1,4,5,*2] ;
机构： [1]Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China [2]Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China [3]Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China [4]Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong University of Technology, Guangdong, Guangzhou 510520, China [5]Zhuhai MUST Science and Technology Research Institute, Guangdong, Zhuhai 51900, China
出处：
DOI： 10.1016/j.biopha.2024.116614
ISSN：

关键词： Pseudolaric acid B Non-small cell lung cancer Reactive oxygen species Autophagy Apoptosis Adenosine 5’-monophosphate-activated protein kinase

摘要：
Pseudolaric acid B (PAB), an acid isolated from the roots of Pseudolarix kaempferi gorden, has shown antitumour effects through multiple mechanisms of action. The objective of this study was to investigate the anticancer effect of PAB on non-small cell lung cancer (NSCLC) and its underlying mechanism. In our experiments, we observed that PAB decreased cell viability, inhibited colony formation, induced cell cycle arrest, impeded scratch healing, and increased apoptosis in H1975 and H1650 cells. Additionally, PAB treatment enhanced the fluorescence intensity of MDC staining in NSCLC cells, upregulated the protein expression of microtubule-associated protein light chain 3 II (LC3 II), and downregulated the expression of sequestosome 1 (SQSTM1/P62). Combined treatment with PAB and chloroquine (CQ) increased the protein expression levels of LC3 II and P62 while decreasing the apoptosis of H1975 and H1650 cells. Moreover, treatment with PAB led to significant mTOR inhibition and AMPK activation. PAB combined with compound C (CC) inhibited autophagy and apoptosis. Furthermore, PAB treatment increased intracellular reactive oxygen species (ROS) levels in NSCLC cells, which correlated with the modulation of the AMPK/mTOR signalling pathway and was associated with autophagy and apoptosis. Finally, we validated the antitumour growth activity and mechanism of PAB in vivo using athymic nude mice bearing H1975 tumour cells. In conclusion, our findings suggest that PAB can induce apoptosis and autophagic cell death in NSCLC through the ROS-triggered AMPK/mTOR signalling pathway, making it a promising candidate for future NSCLC treatment.Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

基金：
This study was funded by the Natural Science Foundation of Sichuan Province (No. 2022NSFSC0046), the Sichuan Science and Technology Program (No. 2022YFS0631), the Sichuan Province Science and Technology Innovation Seedling Project Funding Project (No. MZGC20230038), the Southwest Medical University School-level Scientific Research Project (No. 2021ZKQN095), the Science and Technology Development Fund, Macau SAR (No. 0098/2021/A2 and 0048/ 2023/AFJ), and the Science and Technology Planning Project of Guangdong Province (No. 2020B1212030008).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2024]版：
无
最新[2023]版：
大类 | 2 区 医学
小类 | 1 区 药学 2 区 医学：研究与实验
第一作者：
第一作者机构： [1]Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China [2]Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China [3]Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China
共同第一作者：
通讯作者：
通讯机构： [1]Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China [2]Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China [3]Inflammation & Allergic Diseases Research Unit, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China [4]Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong University of Technology, Guangdong, Guangzhou 510520, China [5]Zhuhai MUST Science and Technology Research Institute, Guangdong, Zhuhai 51900, China [*1]Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646099, China [*2]Faculty of Chinese Medicine, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China.
推荐引用方式(GB/T 7714)：
Luo Dan,He Fang,Liu Jingyun,et al.Pseudolaric acid B suppresses NSCLC progression through the ROS/AMPK/mTOR/autophagy signalling pathway[J].BIOMEDICINE & PHARMACOTHERAPY.2024,175:116614.doi:10.1016/j.biopha.2024.116614.
APA：
Luo Dan,He Fang,Liu Jingyun,Dong Xueting,Fang Mengying...&Wu Qibiao.(2024).Pseudolaric acid B suppresses NSCLC progression through the ROS/AMPK/mTOR/autophagy signalling pathway.BIOMEDICINE & PHARMACOTHERAPY,175,
MLA：
Luo Dan,et al."Pseudolaric acid B suppresses NSCLC progression through the ROS/AMPK/mTOR/autophagy signalling pathway".BIOMEDICINE & PHARMACOTHERAPY 175.(2024):116614

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