机构：[1]Oujiang Laboratory[2] Key Laboratory of Alzheimer's Disease of Zhejiang Province, Institute of Aging, Wenzhou Medical University, Wenzhou, Zhejiang 325024, People's Republic of China.[2]Cancer Centre, Faculty of Health Sciences, MOE Frontier Science Centre for Precision Oncology, University of Macau, Macau SAR 999078, People's Republic of China.[3]Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, People's Republic of China.[4]Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada.[5]State Key Laboratory of Oncogenes and Related Genes, Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
The tumor microenvironment (TME) is critical for tumor growth and metastasis. The TME contains cancer-associated cells, tumor matrix, and tumor secretory factors. The fabrication of artificial tumors, so-called tumoroids, is of great significance for the understanding of tumorigenesis and clinical cancer therapy. The assembly of multiple tumor cells and matrix components through interdisciplinary techniques is necessary for the preparation of various tumoroids. This article discusses current methods for constructing tumoroids (tumor tissue slices and tumor cell co-culture) for pre-clinical use. This article focuses on the artificial matrix materials (natural and synthetic materials) and biofabrication techniques (cell assembly, bioengineered tools, bioprinting, and microfluidic devices) used in tumoroids. This article also points out the shortcomings of current tumoroids and potential solutions. This article aims to promotes the next-generation tumoroids and the potential of them in basic research and clinical application.Creative Commons Attribution license.
基金：
Ministry of Science and Technology of China
(2022YFA1105101); the Chinese Academy of Sciences
(172644KYSB20200002 and 172644KYSB20200048);
the Department of Science and Technology of
Guangdong Province (2021A0505030055); Zhejiang
Provincial Natural Science Foundation of China
(LZ23C070004). YCL thanks the support from the
Chinese Academy of Sciences (Taiwan Young Talent
Program: 2019TW2GB0005). P C thanks the support from the Fund of Southwest Medical University
(2021ZKMS024), and the Science and Technology
Development Fund, Macau SAR (0011/2019/AKP)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|2 区医学
小类|2 区工程：生物医学2 区材料科学：生物材料
最新[2023]版：
大类|2 区医学
小类|2 区工程：生物医学2 区材料科学：生物材料
第一作者：
第一作者机构：[1]Oujiang Laboratory
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Yung-Chiang,Chen Ping,Chang Ray,et al.Artificial tumor matrices and bioengineered tools for tumoroid generation[J].Biofabrication.2024,16(2):doi:10.1088/1758-5090/ad2534.
APA：
Liu Yung-Chiang,Chen Ping,Chang Ray,Liu Xingjian,Jhang Jhe-Wei...&Wang Peng-Yuan.(2024).Artificial tumor matrices and bioengineered tools for tumoroid generation.Biofabrication,16,(2)
MLA：
Liu Yung-Chiang,et al."Artificial tumor matrices and bioengineered tools for tumoroid generation".Biofabrication 16..2(2024)
